Fast determination of saturation intensity and maximum emission rate by single-emitter imaging

by: JYP Butter, B Hecht

Optics Express, Vol. 14 (October 2006), pp. 9350-9357.

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Abstract

We investigate the dependence of the spot size in single-emitter confocal imaging on the degree of saturation. We show that single-emitter spots are broadened and flattened significantly already at excitation intensities well below saturation. The resulting single-emitter spot shapes thus deviate significantly from the excitation point spread function.We show and support by Monte Carlo simulations that fitting of a single spot is sufficient to extract the saturation intensity and the maximum emission rate of a single emitter with high accuracy. Our results will be of interest in all areas of single-emitter studies.

@article{citeulike:1555260, abstract = {We investigate the dependence of the spot size in single-emitter confocal imaging on the degree of saturation. We show that single-emitter spots are broadened and flattened significantly already at excitation intensities well below saturation. The resulting single-emitter spot shapes thus deviate significantly from the excitation point spread function.We show and support by Monte Carlo simulations that fitting of a single spot is sufficient to extract the saturation intensity and the maximum emission rate of a single emitter with high accuracy. Our results will be of interest in all areas of single-emitter studies.}, author = {Butter, J. Y. P. and Hecht, B. }, citeulike-article-id = {1555260}, journal = {Optics Express}, month = {October}, pages = {9350--9357}, posted-at = {2007-08-12 05:44:44}, priority = {2}, title = {Fast determination of saturation intensity and maximum emission rate by single-emitter imaging}, url = {http://adsabs.harvard.edu/cgi-bin/nph-bib\_query?bibcode=2006OExpr..14.9350B}, volume = {14}, year = {2006} }

RIS record

TY - JOUR ID - citeulike:1555260 L3 - citeulike-article-id:1555260 TI - Fast determination of saturation intensity and maximum emission rate by single-emitter imaging JF - Optics Express VL - 14 SP - 9350 EP - 9357 N2 - We investigate the dependence of the spot size in single-emitter confocal imaging on the degree of saturation. We show that single-emitter spots are broadened and flattened significantly already at excitation intensities well below saturation. The resulting single-emitter spot shapes thus deviate significantly from the excitation point spread function.We show and support by Monte Carlo simulations that fitting of a single spot is sufficient to extract the saturation intensity and the maximum emission rate of a single emitter with high accuracy. Our results will be of interest in all areas of single-emitter studies. AU - Butter, JYP AU - Hecht, B PY - 2006/October// UR - http://adsabs.harvard.edu/cgi-bin/nph-bib_query?bibcode=2006OExpr..14.9350B ER -

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