Thu, 24 Jul 2008 23:24:33 BST CiteULike: dchens Ballauff http://www.citeulike.org/user/dchen/author/Ballauff CiteULike.org en-gb Copyright © 2004-2008 citeulike.org http://www.citeulike.org/user/dchen/article/2762175 <i>Physical Review Letters, Vol. 100, No. 15. (2008)</i><br /><br />We present the first time-resolved investigation of motions of proteins in densely grafted layers of spherical polyelectrolyte brushes. Using small-angle x-ray scattering combined with rapid stopped-flow mixing, we followed the uptake of bovine serum albumin by poly(acrylic acid) layer with high spatial and temporal resolution. We find that the total amount of adsorbed protein scales with time as t1/4. This subdiffusive behavior is explained on the basis of directed motion of the protein along the polyelectrolyte chains. Directed Motion of Proteins along Tethered Polyelectrolytes Katja Henzler Sabine Rosenfeldt Alexander Wittemann Ludger Harnau Stephanie Finet Theyencheri Narayanan Matthias Ballauff doi:10.1103/PhysRevLett.100.158301 Physical Review Letters, Vol. 100, No. 15. (2008) 2008-05-06T17:57:50-00:00 2008 Physical Review Letters 100 15 APS 2008 biology diffusion polymer