Thu, 21 Aug 2008 11:04:53 BST CiteULike: sarus Mamillapalli http://www.citeulike.org/user/saru/author/Mamillapalli CiteULike.org en-gb Copyright © 2004-2008 citeulike.org http://www.citeulike.org/user/saru/article/2387788 <i>Cell, Vol. 132, No. 3. (8 February 2008), pp. 449-462.</i><br /><br />Summary Plant innate immunity relies on the recognition of pathogen effector molecules by nucleotide-binding-leucine-rich repeat (NB-LRR) immune receptor families. Previously we have shown the N immune receptor, a member of TIR-NB-LRR family, indirectly recognizes the 50 kDa helicase (p50) domain of Tobacco mosaic virus (TMV) through its TIR domain. We have identified an N receptor-interacting protein, NRIP1, that directly interacts with both N's TIR domain and p50. NRIP1 is a functional rhodanese sulfurtransferase and is required for N to provide complete resistance to TMV. Interestingly, NRIP1 that normally localizes to the chloroplasts is recruited to the cytoplasm and nucleus by the p50 effector. As a consequence, NRIP1 interacts with N only in the presence of the p50 effector. Our findings show that a chloroplastic protein is intimately involved in pathogen recognition. We propose that N's activation requires a prerecognition complex containing the p50 effector and NRIP1. Chloroplastic Protein NRIP1 Mediates Innate Immune Receptor Recognition of a Viral Effector Jeffrey Caplan Padmavathi Mamillapalli Tessa Burch-Smith Kirk Czymmek SP Dinesh-Kumar doi:10.1016/j.cell.2007.12.031 Cell, Vol. 132, No. 3. (8 February 2008), pp. 449-462. 2008-02-16T02:25:25-00:00 2008 Cell 132 3 449 462 n nrip r