Thu, 21 Aug 2008 07:42:56 BST CiteULike: yavannas Friedman http://www.citeulike.org/user/yavanna/author/Friedman CiteULike.org en-gb Copyright © 2004-2008 citeulike.org http://www.citeulike.org/user/yavanna/article/2236570 <i>Proceedings of the National Academy of Sciences, Vol. 105, No. 2. (15 January 2008), pp. 698-703.</i><br /><br />A cell delivery strategy was investigated that was hypothesized to enable magnetic targeting of endothelial cells to the steel surfaces of intraarterial stents because of the following mechanisms: (i) preloading cells with biodegradable polymeric superparamagnetic nanoparticles (MNPs), thereby rendering the cells magnetically responsive; and (ii) the induction of both magnetic field gradients around the wires of a steel stent and magnetic moments within MNPs because of a uniform external magnetic field, thereby targeting MNP-laden cells to the stent wires. In vitro studies demonstrated that MNP-loaded bovine aortic endothelial cells (BAECs) could be magnetically targeted to steel stent wires. In vivo MNP-loaded BAECs transduced with adenoviruses expressing luciferase (Luc) were targeted to stents deployed in rat carotid arteries in the presence of a uniform magnetic field with significantly greater Luc expression, detected by in vivo optical imaging, than nonmagnetic controls. 10.1073/pnas.0708338105 High field gradient targeting of magnetic nanoparticle-loaded endothelial cells to the surfaces of steel stents Boris Polyak Ilia Fishbein Michael Chorny Ivan Alferiev Darryl Williams Ben Yellen Gary Friedman Robert Levy doi:10.1073/pnas.0708338105 Proceedings of the National Academy of Sciences, Vol. 105, No. 2. (15 January 2008), pp. 698-703. 2008-01-15T22:39:52-00:00 2008 Proceedings of the National Academy of Sciences 105 2 698 703 endothelial_cell magnetic medical_science