Thu, 21 Aug 2008 07:39:40 BST CiteULike: yoanjacquemins Allawi http://www.citeulike.org/user/yoanjacquemin/author/Allawi CiteULike.org en-gb Copyright © 2004-2008 citeulike.org http://www.citeulike.org/user/yoanjacquemin/article/100059 <i>Biochemistry, Vol. 35, No. 11. (19 March 1996), pp. 3555-3562.</i><br /><br />Thermodynamic data were determined from UV absorbance vs temperature profiles of 23 oligonucleotides. These data were combined with data from the literature for 21 sequences to derive improved parameters for the 10 Watson-Crick nearest neighbors. The observed trend in nearest-neighbor stabilities at 37 degrees C is GC > CG > GG > GA approximately GT approximately CA > CT > AA > AT > TA (where only the top strand is shown for each nearest neighbor). This trend suggests that both sequence and base composition are important determinants of DNA duplex stability. On average, the improved parameters predict deltaG degrees(37), deltaH degrees, deltaS degrees, and T(m) within 4%, 7%, 8%, and 2 degrees C, respectively. The parameters are optimized for the prediction of oligonucleotides dissolved in 1 M NaC1. Improved nearest-neighbor parameters for predicting DNA duplex stability. J SantaLucia HT Allawi PA Seneviratne doi:10.1021/bi951907q Biochemistry, Vol. 35, No. 11. (19 March 1996), pp. 3555-3562. 2005-02-21T16:22:36-00:00 1996 Biochemistry 0006-2960 35 11 3555 3562 array probe