Rapid and Selective Remodeling of a Positioned Nucleosome during the Induction of IL-12 p40 Transcription

@article{citeulike:1447284, abstract = {Nucleosomes are important for gene regulation, but comprehensive studies of nucleosome positioning, remodeling, and transcription factor binding at inducible mammalian promoters have not been reported. We have analyzed the IL-12 p40 promoter, which is induced in macrophages by bacterial products. High-resolution micrococcal nuclease analyses revealed that a positioned nucleosome, nucleosome 1, spans the promoter, with three positioned nucleosomes further upstream. Upon activation, nucleosome 1 was rapidly and selectively remodeled in a protein synthesis-dependent manner. In primary macrophages, IFN[gamma] synergistically enhanced p40 expression, but little effect on remodeling or promoter occupancy was observed. These results suggest that remodeling complexes are selectively targeted to a single, promoter-encompassing nucleosome and that IFN[gamma] influences an event that is independent or downstream of remodeling.}, author = {Weinmann, Amy S. and Plevy, Scott E. and Smale, Stephen T. }, citeulike-article-id = {1447284}, doi = {10.1016/S1074-7613(00)80141-7}, journal = {Immunity}, keywords = {il-12-general}, month = {December}, number = {6}, pages = {665--675}, posted-at = {2007-07-10 21:20:43}, priority = {2}, title = {Rapid and Selective Remodeling of a Positioned Nucleosome during the Induction of IL-12 p40 Transcription}, url = {http://dx.doi.org/10.1016/S1074-7613(00)80141-7}, volume = {11}, year = {1999} }

TY - JOUR ID - citeulike:1447284 L3 - citeulike-article-id:1447284 TI - Rapid and Selective Remodeling of a Positioned Nucleosome during the Induction of IL-12 p40 Transcription JF - Immunity VL - 11 IS - 6 SP - 665 EP - 675 N2 - Nucleosomes are important for gene regulation, but comprehensive studies of nucleosome positioning, remodeling, and transcription factor binding at inducible mammalian promoters have not been reported. We have analyzed the IL-12 p40 promoter, which is induced in macrophages by bacterial products. High-resolution micrococcal nuclease analyses revealed that a positioned nucleosome, nucleosome 1, spans the promoter, with three positioned nucleosomes further upstream. Upon activation, nucleosome 1 was rapidly and selectively remodeled in a protein synthesis-dependent manner. In primary macrophages, IFN[gamma] synergistically enhanced p40 expression, but little effect on remodeling or promoter occupancy was observed. These results suggest that remodeling complexes are selectively targeted to a single, promoter-encompassing nucleosome and that IFN[gamma] influences an event that is independent or downstream of remodeling. KW - il-12-general AU - Weinmann, Amy AU - Plevy, Scott AU - Smale, Stephen PY - 1999/12/01/ UR - http://dx.doi.org/10.1016/S1074-7613(00)80141-7 ER -