IL-10 Gene Expression Is Controlled by the Transcription Factors Sp1 and Sp3

@article{citeulike:1452644, abstract = {IL-10 is an 18-kDa cytokine with a key role in homeostatic control of inflammatory and immune responses. We have investigated how transcription of the IL-10 gene is regulated, so as to be able to understand the circumstances of IL-10 expression in both health and disease. In the mouse, IL-10 gene expression is regulated by a TATA-type promoter with a critical cis-acting element containing GGA repeats located at -89 to -77. Its complementary sequence is similar to the cis-acting elements (TCC repeats) in the promoters of genes encoding epidermal growth factor receptor and CD58. All these elements comprise a common CCTCCT sequence with less conserved C + T-rich sequences. Eliminating this CCTCCT sequence results in a marked reduction in promoter activity, suggesting a necessary role in IL-10 gene expression. Despite its dissimilarity to the G + C-rich Sp1 consensus sequence (GC box), Sp1 and Sp3 transcription factors could be shown to bind to this motif. The requirement for Sp1 and Sp3 in transcription of IL-10 was confirmed using Drosophila SL2 cells, which lack endogenous Sp factors. These results suggest that the transcription of IL-10 is positively regulated by both Sp1 and Sp3.}, author = {Tone, Masahide and Powell, Mark J. and Tone, Yukiko and Thompson, Sara A. and Waldmann, Herman }, citeulike-article-id = {1452644}, journal = {J Immunol}, keywords = {il-10-general}, month = {July}, number = {1}, pages = {286--291}, posted-at = {2007-07-12 16:33:56}, priority = {2}, title = {IL-10 Gene Expression Is Controlled by the Transcription Factors Sp1 and Sp3}, url = {http://www.jimmunol.org/cgi/content/abstract/165/1/286}, volume = {165}, year = {2000} }

TY - JOUR ID - citeulike:1452644 L3 - citeulike-article-id:1452644 TI - IL-10 Gene Expression Is Controlled by the Transcription Factors Sp1 and Sp3 JF - J Immunol VL - 165 IS - 1 SP - 286 EP - 291 N2 - IL-10 is an 18-kDa cytokine with a key role in homeostatic control of inflammatory and immune responses. We have investigated how transcription of the IL-10 gene is regulated, so as to be able to understand the circumstances of IL-10 expression in both health and disease. In the mouse, IL-10 gene expression is regulated by a TATA-type promoter with a critical cis-acting element containing GGA repeats located at -89 to -77. Its complementary sequence is similar to the cis-acting elements (TCC repeats) in the promoters of genes encoding epidermal growth factor receptor and CD58. All these elements comprise a common CCTCCT sequence with less conserved C + T-rich sequences. Eliminating this CCTCCT sequence results in a marked reduction in promoter activity, suggesting a necessary role in IL-10 gene expression. Despite its dissimilarity to the G + C-rich Sp1 consensus sequence (GC box), Sp1 and Sp3 transcription factors could be shown to bind to this motif. The requirement for Sp1 and Sp3 in transcription of IL-10 was confirmed using Drosophila SL2 cells, which lack endogenous Sp factors. These results suggest that the transcription of IL-10 is positively regulated by both Sp1 and Sp3. KW - il-10-general AU - Tone, Masahide AU - Powell, Mark AU - Tone, Yukiko AU - Thompson, Sara AU - Waldmann, Herman PY - 2000/07/01/ UR - http://www.jimmunol.org/cgi/content/abstract/165/1/286 ER -