In Situ Expression of Interleukin-10 in Noninflamed Human Gut and in Inflammatory Bowel Disease

@article{citeulike:1507575, abstract = {A dysregulated secretion of contra-inflammatory cytokines such as interleukin-10 (IL-10) could play a role in the pathogenesis of inflammatory bowel disease (IBD). We have investigated the expression of IL-10 in gut tissues from patients with Crohn's disease (CD), ulcerative colitis (UC) and controls by mRNA in situ hybridization and immunohistochemistry. Intestinal epithelial cells were found to express IL-10 mRNA and IL-10 protein in all of the tissues investigated without any major differences in the expression patterns. However, compared with noninflamed gut, significantly increased numbers of mononuclear cells (MNCs) producing IL-10 were present in inflamed gut, both in CD and UC. This cytokine was expressed most prominently by inflammatory infiltrates enriched in macrophages, although T cells seem to contribute to its production as well. Elevated IL-10 expression in IBD was mainly detected in the submucosa, whereas IL-10 production by lamina propria cells remained comparably low. In contrast, the expression of IL-1{beta} mRNA was preferentially increased in the lamina propria. Our data argue against a general deficiency in IL-10 production in IBD. The results suggest rather that the local production of IL-10 by mucosal MNCs in IBD is insufficient to down-regulate pro-inflammatory cytokines such as IL-1{beta} in the lamina propria compartment.}, author = {Autschbach, Frank and Braunstein, Jutta and Helmke, Burkhard and Zuna, Ivan and Schurmann, Guido and Niemir, Zofia I. and Wallich, Reinhard and Otto, Herwart F. and Meuer, Stefan C. }, citeulike-article-id = {1507575}, journal = {Am J Pathol}, keywords = {il-10-gut}, month = {July}, number = {1}, pages = {121--130}, posted-at = {2007-07-27 19:57:07}, priority = {2}, title = {In Situ Expression of Interleukin-10 in Noninflamed Human Gut and in Inflammatory Bowel Disease}, url = {http://ajp.amjpathol.org/cgi/content/abstract/153/1/121}, volume = {153}, year = {1998} }

TY - JOUR ID - citeulike:1507575 L3 - citeulike-article-id:1507575 TI - In Situ Expression of Interleukin-10 in Noninflamed Human Gut and in Inflammatory Bowel Disease JF - Am J Pathol VL - 153 IS - 1 SP - 121 EP - 130 N2 - A dysregulated secretion of contra-inflammatory cytokines such as interleukin-10 (IL-10) could play a role in the pathogenesis of inflammatory bowel disease (IBD). We have investigated the expression of IL-10 in gut tissues from patients with Crohn's disease (CD), ulcerative colitis (UC) and controls by mRNA in situ hybridization and immunohistochemistry. Intestinal epithelial cells were found to express IL-10 mRNA and IL-10 protein in all of the tissues investigated without any major differences in the expression patterns. However, compared with noninflamed gut, significantly increased numbers of mononuclear cells (MNCs) producing IL-10 were present in inflamed gut, both in CD and UC. This cytokine was expressed most prominently by inflammatory infiltrates enriched in macrophages, although T cells seem to contribute to its production as well. Elevated IL-10 expression in IBD was mainly detected in the submucosa, whereas IL-10 production by lamina propria cells remained comparably low. In contrast, the expression of IL-1{beta} mRNA was preferentially increased in the lamina propria. Our data argue against a general deficiency in IL-10 production in IBD. The results suggest rather that the local production of IL-10 by mucosal MNCs in IBD is insufficient to down-regulate pro-inflammatory cytokines such as IL-1{beta} in the lamina propria compartment. KW - il-10-gut AU - Autschbach, Frank AU - Braunstein, Jutta AU - Helmke, Burkhard AU - Zuna, Ivan AU - Schurmann, Guido AU - Niemir, Zofia AU - Wallich, Reinhard AU - Otto, Herwart AU - Meuer, Stefan PY - 1998/07/01/ UR - http://ajp.amjpathol.org/cgi/content/abstract/153/1/121 ER -