Regulatory dendritic cells act as regulators of acute lethal systemic inflammatory response

@article{citeulike:1525717, abstract = {Bacterial infection triggers host inflammation through the activation of immune cells, leading to the elimination of bacteria. However, the regulatory mechanisms of the host inflammatory response remain unknown. Here we report that a subset of potent tolerogenic dendritic cells (DCs), regulatory DCs (DCregs), control the systemic inflammatory response. Unlike normal DCs, which produced proinflammatory cytokines in response to bacterial lipopolysaccharide (LPS), DCregs produced fewer proinflammatory cytokines and instead preferentially produced interleukin-10 (IL-10), and these events involved the expression of I{kappa}BNS and Bcl-3 as well as cyclic AMP (cAMP)-mediated activation of protein kinase A (PKA). In addition, DCregs not only suppressed LPS-induced production of proinflammatory cytokines in macrophages, but also reduced their serum levels in mice. Furthermore, DCregs protected mice against the lethality induced by experimental endotoxemia and bacterial peritonitis. The inhibitory effect of DCregs against inflammatory responses involved the production of IL-10. On the other hand, naturally existing tolerogenic DC subsets producing IL-10, CD11clowCD45RBhigh DCs, also suppressed LPS-induced host inflammatory responses. Thus, a subset of tolerogenic DCs act as potential regulators of the host inflammatory response, and they might have preventive and therapeutic potential for the treatment of systemic as well as local inflammatory diseases. 10.1182/blood-2005-10-4190}, author = {Fujita, Shigeharu and Seino, Ken-Ichiro and Sato, Kaori and Sato, Yumiko and Eizumi, Kawori and Yamashita, Naohide and Taniguchi, Masaru and Sato, Katsuaki }, citeulike-article-id = {1525717}, doi = {10.1182/blood-2005-10-4190}, journal = {Blood}, keywords = {tolerogenic-dcs}, month = {May}, number = {9}, pages = {3656--3664}, posted-at = {2007-07-31 21:43:50}, priority = {2}, title = {Regulatory dendritic cells act as regulators of acute lethal systemic inflammatory response}, url = {http://dx.doi.org/10.1182/blood-2005-10-4190}, volume = {107}, year = {2006} }

TY - JOUR ID - citeulike:1525717 L3 - citeulike-article-id:1525717 TI - Regulatory dendritic cells act as regulators of acute lethal systemic inflammatory response JF - Blood VL - 107 IS - 9 SP - 3656 EP - 3664 N2 - Bacterial infection triggers host inflammation through the activation of immune cells, leading to the elimination of bacteria. However, the regulatory mechanisms of the host inflammatory response remain unknown. Here we report that a subset of potent tolerogenic dendritic cells (DCs), regulatory DCs (DCregs), control the systemic inflammatory response. Unlike normal DCs, which produced proinflammatory cytokines in response to bacterial lipopolysaccharide (LPS), DCregs produced fewer proinflammatory cytokines and instead preferentially produced interleukin-10 (IL-10), and these events involved the expression of I{kappa}BNS and Bcl-3 as well as cyclic AMP (cAMP)-mediated activation of protein kinase A (PKA). In addition, DCregs not only suppressed LPS-induced production of proinflammatory cytokines in macrophages, but also reduced their serum levels in mice. Furthermore, DCregs protected mice against the lethality induced by experimental endotoxemia and bacterial peritonitis. The inhibitory effect of DCregs against inflammatory responses involved the production of IL-10. On the other hand, naturally existing tolerogenic DC subsets producing IL-10, CD11clowCD45RBhigh DCs, also suppressed LPS-induced host inflammatory responses. Thus, a subset of tolerogenic DCs act as potential regulators of the host inflammatory response, and they might have preventive and therapeutic potential for the treatment of systemic as well as local inflammatory diseases. 10.1182/blood-2005-10-4190 KW - tolerogenic-dcs AU - Fujita, Shigeharu AU - Seino, Ken-Ichiro AU - Sato, Kaori AU - Sato, Yumiko AU - Eizumi, Kawori AU - Yamashita, Naohide AU - Taniguchi, Masaru AU - Sato, Katsuaki PY - 2006/05/01/ UR - http://dx.doi.org/10.1182/blood-2005-10-4190 ER -