Characterization of Dendritic Cells that Induce Tolerance and T Regulatory 1 Cell Differentiation In Vivo

@article{citeulike:1525719, abstract = {Active suppression is mediated by a subpopulation of CD4+ T cells that prevents autoimmunity. However, the mechanisms involved in their differentiation in vivo are currently under intensive research. Here we show that in vitro culture of bone marrow cells in the presence of IL-10 induces the differentiation of a distinct subset of dendritic cells with a specific expression of CD45RB. These CD11clowCD45RBhigh DCs are present in the spleen and lymph nodes of normal mice and are significantly enriched in the spleen of IL-10 Tg mice. These natural or in vitro-derived DCs display plasmacytoid morphology and an immature-like phenotype, and secrete high levels of IL-10 after activation. OVA peptide-pulsed CD11clowCD45RBhigh DCs specifically induce tolerance through the differentiation of Tr1 cells in vitro and in vivo. Our findings identify a natural DC subset that induces the differentiation of Tr1 cells and suggest their therapeutic use.}, author = {Wakkach, Abdelilah and Fournier, Nathalie and Brun, Valerie and Breittmayer, Jean-Philippe and Cottrez, Francoise and Groux, Herve }, citeulike-article-id = {1525719}, doi = {10.1016/S1074-7613(03)00113-4}, journal = {Immunity}, keywords = {tolerogenic-dcs}, month = {May}, number = {5}, pages = {605--617}, posted-at = {2007-07-31 21:45:39}, priority = {2}, title = {Characterization of Dendritic Cells that Induce Tolerance and T Regulatory 1 Cell Differentiation In Vivo}, url = {http://dx.doi.org/10.1016/S1074-7613(03)00113-4}, volume = {18}, year = {2003} }

TY - JOUR ID - citeulike:1525719 L3 - citeulike-article-id:1525719 TI - Characterization of Dendritic Cells that Induce Tolerance and T Regulatory 1 Cell Differentiation In Vivo JF - Immunity VL - 18 IS - 5 SP - 605 EP - 617 N2 - Active suppression is mediated by a subpopulation of CD4+ T cells that prevents autoimmunity. However, the mechanisms involved in their differentiation in vivo are currently under intensive research. Here we show that in vitro culture of bone marrow cells in the presence of IL-10 induces the differentiation of a distinct subset of dendritic cells with a specific expression of CD45RB. These CD11clowCD45RBhigh DCs are present in the spleen and lymph nodes of normal mice and are significantly enriched in the spleen of IL-10 Tg mice. These natural or in vitro-derived DCs display plasmacytoid morphology and an immature-like phenotype, and secrete high levels of IL-10 after activation. OVA peptide-pulsed CD11clowCD45RBhigh DCs specifically induce tolerance through the differentiation of Tr1 cells in vitro and in vivo. Our findings identify a natural DC subset that induces the differentiation of Tr1 cells and suggest their therapeutic use. KW - tolerogenic-dcs AU - Wakkach, Abdelilah AU - Fournier, Nathalie AU - Brun, Valerie AU - Breittmayer, Jean-Philippe AU - Cottrez, Francoise AU - Groux, Herve PY - 2003/05// UR - http://dx.doi.org/10.1016/S1074-7613(03)00113-4 ER -