Shinella kummerowiae sp. nov., a symbiotic bacterium isolated from root nodules of the herbal legume Kummerowia stipulacea

@article{citeulike:2874331, abstract = {Bacterial strain CCBAU 25048T was isolated from root nodules of Kummerowia stipulacea grown in Shandong province of China. Cells of the strain were Gram-negative, strictly aerobic, non-spore-forming, motile short rods. Phylogeny of 16S rRNA gene sequences revealed that the strain belonged to the genus Shinella, a member of family Rhizobiaceae. Its closest phylogenetic relatives were Shinella granuli Ch06T and Shinella zoogloeoides IAM 12669T, respectively showing 98.3 and 98.9 \% 16S rRNA gene sequence similarity. Strain CCBAU 25048T had DNA-DNA relatedness of 43.5 and 34.8 \%, respectively, with S. zoogloeoides JCM 20728T and S. granuli JCM 13254T. In addition, in TP-RAPD analysis, different patterns were obtained for these three strains and some rhizobial strains. The nifH, nodC and nodD sequences of CCBAU 25048T were identical or very similar to those of bean-nodulating Rhizobium tropici strains. Several phenotypic characteristics, including the use of citrate and D-ribose as carbon sources and growth at pH 11.0, as well as the fatty acid composition, could differentiate CCBAU 25048T from the two defined Shinella species. Therefore, a novel species Shinella kummerowiae sp. nov. is proposed, with strain CCBAU 25048T (=JCM 14778T =LMG 24136T) as the type strain. 10.1099/ijs.0.65723-0}, author = {Lin, Dong X. and Wang, En T. and Tang, Hui and Han, Tian X. and He, Yu R. and Guan, Su H. and Chen, Wen X. }, citeulike-article-id = {2874331}, doi = {http://dx.doi.org/10.1099/ijs.0.65723-0}, journal = {Int J Syst Evol Microbiol}, keywords = {new, rhizobia}, month = {June}, number = {6}, pages = {1409--1413}, posted-at = {2008-06-09 04:18:35}, priority = {5}, title = {Shinella kummerowiae sp. nov., a symbiotic bacterium isolated from root nodules of the herbal legume Kummerowia stipulacea}, url = {http://dx.doi.org/10.1099/ijs.0.65723-0}, volume = {58}, year = {2008} }

TY - JOUR ID - citeulike:2874331 L3 - citeulike-article-id:2874331 TI - Shinella kummerowiae sp. nov., a symbiotic bacterium isolated from root nodules of the herbal legume Kummerowia stipulacea JF - Int J Syst Evol Microbiol VL - 58 IS - 6 SP - 1409 EP - 1413 N2 - Bacterial strain CCBAU 25048T was isolated from root nodules of Kummerowia stipulacea grown in Shandong province of China. Cells of the strain were Gram-negative, strictly aerobic, non-spore-forming, motile short rods. Phylogeny of 16S rRNA gene sequences revealed that the strain belonged to the genus Shinella, a member of family Rhizobiaceae. Its closest phylogenetic relatives were Shinella granuli Ch06T and Shinella zoogloeoides IAM 12669T, respectively showing 98.3 and 98.9 % 16S rRNA gene sequence similarity. Strain CCBAU 25048T had DNA-DNA relatedness of 43.5 and 34.8 %, respectively, with S. zoogloeoides JCM 20728T and S. granuli JCM 13254T. In addition, in TP-RAPD analysis, different patterns were obtained for these three strains and some rhizobial strains. The nifH, nodC and nodD sequences of CCBAU 25048T were identical or very similar to those of bean-nodulating Rhizobium tropici strains. Several phenotypic characteristics, including the use of citrate and D-ribose as carbon sources and growth at pH 11.0, as well as the fatty acid composition, could differentiate CCBAU 25048T from the two defined Shinella species. Therefore, a novel species Shinella kummerowiae sp. nov. is proposed, with strain CCBAU 25048T (=JCM 14778T =LMG 24136T) as the type strain. 10.1099/ijs.0.65723-0 KW - new KW - rhizobia AU - Lin, Dong AU - Wang, En AU - Tang, Hui AU - Han, Tian AU - He, Yu AU - Guan, Su AU - Chen, Wen PY - 2008/06/01/ UR - http://dx.doi.org/10.1099/ijs.0.65723-0 ER -