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Rhizobium miluonense sp. nov., a symbiotic bacterium isolated from Lespedeza root nodules

by: Chun T Gu, En T Wang, Chang F Tian, Tian X Han, Wen F Chen, Xin H Sui, Wen X Chen
Int J Syst Evol Microbiol, Vol. 58, No. 6. (1 June 2008), pp. 1364-1368.


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Seventeen strains of lespedeza rhizobia, a previously defined novel group, were further characterized by comparison with phylogenetically related species using a polyphasic approach, including SDS-PAGE of whole-cell proteins, PCR-based restriction fragment length polymorphism analysis of ribosomal intergenic spacer sequences, determination of DNA G+C contents, DNA-DNA hybridization and analysis of phenotypic features. Based on data obtained in the present and previous studies, a novel species, Rhizobium miluonense sp. nov., is proposed for these strains. The type strain of R. miluonense is CCBAU 41251T (=LMG 24208T=HAMBI 2971T). Strain CCBAU 41251T could form ineffective nodules on Phaseolus vulgaris in addition to its original host Lespedeza chinensis. 10.1099/ijs.0.65661-0


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