HMGB1 as a predictor of organ dysfunction and outcome in patients with severe sepsis.

@article{citeulike:2434870, abstract = {OBJECTIVE: To study the predictive value of high mobility group box-1 protein (HMGB1) and hospital mortality in adult patients with severe sepsis. STUDY DESIGN: Prospective observational cohort study in 24 ICUs in Finland. PATIENTS: Two hundred and forty-seven adult patients with severe sepsis. MEASUREMENTS AND MAIN RESULTS: Blood samples for HMGB1 analyses were drawn from 247 patients at baseline and from 210 patients 72[Symbol: see text]h later. The mean APACHE II and SAPS II scores were 24 (SD 9) and 44 (SD 17), respectively. The hospital mortality was 26\%. The serum HMGB1 concentrations were measured first by semi-quantitative Western immunoblotting (WB) analysis. The median HMGB1 concentration on day 0 was 108\% (IQR 98.5-119) and after 72[Symbol: see text]h 107\% (IQR 98.8-120), which differed from healthy controls (97.5\%, IQR 91.3-106.5; p[Symbol: see text]=[Symbol: see text]0.028 and 0.019, respectively). The samples were re-analysed by ELISA (in a subgroup of 170 patients) to confirm the results by WB. The median concentration in healthy controls was 0.65[Symbol: see text]ng/ml (IQR 0.51-1.0). This was lower than in patients with severe sepsis (3.6[Symbol: see text]ng/ml, IQR 1.9-6.5, p[Symbol: see text]<[Symbol: see text]0.001). HMGB1 concentrations (WB and ELISA) did not differ between hospital survivors and non-survivors. In ROC analyses for HMGB1 levels (WB) on day 0 and 72[Symbol: see text]h with respect to hospital mortality, the areas under the curve were 0.51 and 0.56 (95\% CI 0.40-0.61 and 0.47-0.65). CONCLUSIONS: Serum HMGB1 concentrations were elevated in patients with severe sepsis, but did not differ between survivors and non-survivors and did not predict hospital mortality.}, address = {Department of Intensive Care Medicine, Tampere University Hospital, Teiskontie 35, 33521, Tampere, Finland, sari.karlsson@pshp.fi.}, author = {Karlsson, Sari and Pettil\"{a}, Ville and Tenhunen, Jyrki and Laru-Sompa, Raili and Hynninen, Marja and Ruokonen, Esko }, citeulike-article-id = {2434870}, doi = {http://dx.doi.org/10.1007/s00134-008-1032-9}, issn = {0342-4642}, journal = {Intensive Care Med}, month = {February}, posted-at = {2008-02-27 08:08:26}, priority = {2}, title = {HMGB1 as a predictor of organ dysfunction and outcome in patients with severe sepsis.}, url = {http://dx.doi.org/10.1007/s00134-008-1032-9}, year = {2008} }

TY - JOUR ID - citeulike:2434870 L3 - citeulike-article-id:2434870 TI - HMGB1 as a predictor of organ dysfunction and outcome in patients with severe sepsis. JF - Intensive Care Med AD - Department of Intensive Care Medicine, Tampere University Hospital, Teiskontie 35, 33521, Tampere, Finland, sari.karlsson@pshp.fi. SN - 0342-4642 N2 - OBJECTIVE: To study the predictive value of high mobility group box-1 protein (HMGB1) and hospital mortality in adult patients with severe sepsis. STUDY DESIGN: Prospective observational cohort study in 24 ICUs in Finland. PATIENTS: Two hundred and forty-seven adult patients with severe sepsis. MEASUREMENTS AND MAIN RESULTS: Blood samples for HMGB1 analyses were drawn from 247 patients at baseline and from 210 patients 72[Symbol: see text]h later. The mean APACHE II and SAPS II scores were 24 (SD 9) and 44 (SD 17), respectively. The hospital mortality was 26%. The serum HMGB1 concentrations were measured first by semi-quantitative Western immunoblotting (WB) analysis. The median HMGB1 concentration on day 0 was 108% (IQR 98.5-119) and after 72[Symbol: see text]h 107% (IQR 98.8-120), which differed from healthy controls (97.5%, IQR 91.3-106.5; p[Symbol: see text]=[Symbol: see text]0.028 and 0.019, respectively). The samples were re-analysed by ELISA (in a subgroup of 170 patients) to confirm the results by WB. The median concentration in healthy controls was 0.65[Symbol: see text]ng/ml (IQR 0.51-1.0). This was lower than in patients with severe sepsis (3.6[Symbol: see text]ng/ml, IQR 1.9-6.5, p[Symbol: see text]<[Symbol: see text]0.001). HMGB1 concentrations (WB and ELISA) did not differ between hospital survivors and non-survivors. In ROC analyses for HMGB1 levels (WB) on day 0 and 72[Symbol: see text]h with respect to hospital mortality, the areas under the curve were 0.51 and 0.56 (95% CI 0.40-0.61 and 0.47-0.65). CONCLUSIONS: Serum HMGB1 concentrations were elevated in patients with severe sepsis, but did not differ between survivors and non-survivors and did not predict hospital mortality. AU - Karlsson, Sari AU - Pettilä, Ville AU - Tenhunen, Jyrki AU - Laru-Sompa, Raili AU - Hynninen, Marja AU - Ruokonen, Esko PY - 2008/02/23/ UR - http://dx.doi.org/10.1007/s00134-008-1032-9 ER -