Conserved and Differential Effects of Dietary Energy Intake on the Hippocampal Transcriptomes of Females and Males

@article{citeulike:2946391, abstract = {The level of dietary energy intake influences metabolism, reproductive function, the development of age-related diseases, and even cognitive behavior. Because males and females typically play different roles in the acquisition and allocation of energy resources, we reasoned that dietary energy intake might differentially affect the brains of males and females at the molecular level. To test this hypothesis, we performed a gene array analysis of the hippocampus in male and female rats that had been maintained for 6 months on either ad libitum (control), 20\% caloric restriction (CR), 40\% CR, intermittent fasting (IF) or high fat/high glucose (HFG) diets. These diets resulted in expected changes in body weight, and circulating levels of glucose, insulin and leptin. However, the CR diets significantly increased the size of the hippocampus of females, but not males. Multiple genes were regulated coherently in response to energy restriction diets in females, but not in males. Functional physiological pathway analyses showed that the 20\% CR diet down-regulated genes involved in glycolysis and mitochondrial ATP production in males, whereas these metabolic pathways were up-regulated in females. The 40\% CR diet up-regulated genes involved in glycolysis, protein deacetylation, PGC-1α and mTor pathways in both sexes. IF down-regulated many genes in males including those involved in protein degradation and apoptosis, but up-regulated many genes in females including those involved in cellular energy metabolism, cell cycle regulation and protein deacetylation. Genes involved in energy metabolism, oxidative stress responses and cell death were affected by the HFG diet in both males and females. The gender-specific molecular genetic responses of hippocampal cells to variations in dietary energy intake identified in this study may mediate differential behavioral responses of males and females to differences in energy availability.}, author = {Martin, Bronwen and Pearson, Michele and Brenneman, Randall and Golden, Erin and Keselman, Alex and Iyun, Titilola and Carlson, Olga D. and Egan, Josephine M. and Becker, Kevin G. and Wood, William and Prabhu, Vinayakumar and de Cabo, Rafael and Maudsley, Stuart and Mattson, Mark P. }, citeulike-article-id = {2946391}, doi = {http://dx.doi.org/10.1371/journal.pone.0002398}, journal = {PLoS ONE}, keywords = {adaptive-systems, brain-anatomy, cell-models, genome-interactome, genome-transcriptome, molecular-general, neuro-chem, sex-differentiation}, month = {June}, number = {6}, pages = {e2398+}, posted-at = {2008-07-01 04:02:39}, priority = {4}, publisher = {Public Library of Science}, title = {Conserved and Differential Effects of Dietary Energy Intake on the Hippocampal Transcriptomes of Females and Males}, url = {http://dx.doi.org/10.1371/journal.pone.0002398}, volume = {3}, year = {2008} }

TY - JOUR ID - citeulike:2946391 L3 - citeulike-article-id:2946391 TI - Conserved and Differential Effects of Dietary Energy Intake on the Hippocampal Transcriptomes of Females and Males JF - PLoS ONE VL - 3 IS - 6 SP - e2398 PB - Public Library of Science N2 - The level of dietary energy intake influences metabolism, reproductive function, the development of age-related diseases, and even cognitive behavior. Because males and females typically play different roles in the acquisition and allocation of energy resources, we reasoned that dietary energy intake might differentially affect the brains of males and females at the molecular level. To test this hypothesis, we performed a gene array analysis of the hippocampus in male and female rats that had been maintained for 6 months on either ad libitum (control), 20% caloric restriction (CR), 40% CR, intermittent fasting (IF) or high fat/high glucose (HFG) diets. These diets resulted in expected changes in body weight, and circulating levels of glucose, insulin and leptin. However, the CR diets significantly increased the size of the hippocampus of females, but not males. Multiple genes were regulated coherently in response to energy restriction diets in females, but not in males. Functional physiological pathway analyses showed that the 20% CR diet down-regulated genes involved in glycolysis and mitochondrial ATP production in males, whereas these metabolic pathways were up-regulated in females. The 40% CR diet up-regulated genes involved in glycolysis, protein deacetylation, PGC-1α and mTor pathways in both sexes. IF down-regulated many genes in males including those involved in protein degradation and apoptosis, but up-regulated many genes in females including those involved in cellular energy metabolism, cell cycle regulation and protein deacetylation. Genes involved in energy metabolism, oxidative stress responses and cell death were affected by the HFG diet in both males and females. The gender-specific molecular genetic responses of hippocampal cells to variations in dietary energy intake identified in this study may mediate differential behavioral responses of males and females to differences in energy availability. KW - adaptive-systems KW - brain-anatomy KW - cell-models KW - genome-interactome KW - genome-transcriptome KW - molecular-general KW - neuro-chem KW - sex-differentiation AU - Martin, Bronwen AU - Pearson, Michele AU - Brenneman, Randall AU - Golden, Erin AU - Keselman, Alex AU - Iyun, Titilola AU - Carlson, Olga AU - Egan, Josephine AU - Becker, Kevin AU - Wood, William AU - Prabhu, Vinayakumar AU - de Cabo, Rafael AU - Maudsley, Stuart AU - Mattson, Mark PY - 2008/06/11/ UR - http://dx.doi.org/10.1371/journal.pone.0002398 ER -