Heat shock cognate protein 70 is involved in rotavirus cell entry.

@article{citeulike:2967870, abstract = {In this work, we have identified the heat shock cognate protein (hsc70) as a receptor candidate for rotaviruses. hsc70 was shown to be present on the surface of MA104 cells, and antibodies to this protein blocked rotavirus infectivity, while not affecting the infectivity of reovirus and poliovirus. Preincubation of the hsc70 protein with the viruses also inhibited their infectivity. Triple-layered particles (mature virions), but not double-layered particles, bound hsc70 in a solid-phase assay, and this interaction was blocked by monoclonal antibodies to the virus surface proteins VP4 and VP7. Rotaviruses were shown to interact with hsc70 at a postattachment step, since antibodies to hsc70 and the protein itself did not inhibit the virus attachment to cells. We propose that the functional rotavirus receptor is a complex of several cell surface molecules that include, among others, hsc70.}, address = {Departamento de Gen\'{e}tica y Fisiolog\'{i}a Molecular, Instituto de Biotecnolog\'{i}a, Universidad Nacional Aut\'{o}noma de M\'{e}xico, Cuernavaca, Morelos 62250, Mexico, USA.}, author = {Guerrero, C. A. and Bouyssounade, D. and Z\'{a}rate, S. and Isa, P. and L\'{o}pez, T. and Espinosa, R. and Romero, P. and M\'{e}ndez, E. and L\'{o}pez, S. and Arias, C. F. }, citeulike-article-id = {2967870}, issn = {0022-538X}, journal = {Journal of virology}, month = {April}, number = {8}, pages = {4096--4102}, posted-at = {2008-07-06 21:35:13}, priority = {2}, title = {Heat shock cognate protein 70 is involved in rotavirus cell entry.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/11907249}, volume = {76}, year = {2002} }

TY - JOUR ID - citeulike:2967870 L3 - citeulike-article-id:2967870 TI - Heat shock cognate protein 70 is involved in rotavirus cell entry. JF - Journal of virology VL - 76 IS - 8 SP - 4096 EP - 4102 AD - Departamento de Genética y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos 62250, Mexico, USA. SN - 0022-538X N2 - In this work, we have identified the heat shock cognate protein (hsc70) as a receptor candidate for rotaviruses. hsc70 was shown to be present on the surface of MA104 cells, and antibodies to this protein blocked rotavirus infectivity, while not affecting the infectivity of reovirus and poliovirus. Preincubation of the hsc70 protein with the viruses also inhibited their infectivity. Triple-layered particles (mature virions), but not double-layered particles, bound hsc70 in a solid-phase assay, and this interaction was blocked by monoclonal antibodies to the virus surface proteins VP4 and VP7. Rotaviruses were shown to interact with hsc70 at a postattachment step, since antibodies to hsc70 and the protein itself did not inhibit the virus attachment to cells. We propose that the functional rotavirus receptor is a complex of several cell surface molecules that include, among others, hsc70. AU - Guerrero, CA AU - Bouyssounade, D AU - Zárate, S AU - Isa, P AU - López, T AU - Espinosa, R AU - Romero, P AU - Méndez, E AU - López, S AU - Arias, CF PY - 2002/04// UR - http://view.ncbi.nlm.nih.gov/pubmed/11907249 ER -