Pax6 is required for delta-catenin/neurojugin expression during retinal, cerebellar and cortical development in mice.

@article{citeulike:1361163, abstract = {The transcription factor Pax6 regulates multiple aspects of central nervous system (CNS) development. At the cellular level, the Pax6 mutation was reported to affect homophilic and heterophilic cellular adhesion, neuron polarity and neurite outgrowth. These abnormalities were observed in multiple regions of Pax6-mutant CNS, suggesting a common function for Pax6 in regulating cytoskeletal dynamics. However, target genes mediating Pax6 function in cytoskeletal dynamics remain largely unknown. Using DNA microarrays, we identified delta-catenin (delta-catenin /neurojugin) as a potential direct target of Pax6 in the CNS. delta-catenin encodes a large cytoskeletal protein that localizes at adherens junction in the CNS and that can modulate neurite outgrowth and N-cadherin turnover. delta-catenin was found to be co-expressed with Pax6 in several regions of the developing CNS. In Pax6 mutant embryos, delta-catenin expression was severely reduced in the optic vesicle neural ectoderm, in the ventricular zone of the neocortex and in the external granule layer of the cerebellum. We identified a Pax6 binding site in delta-catenin promoter that is conserved between mice and humans and which is effectively bound by Pax6 in vitro. Our results suggest that Pax6 regulates delta-catenin expression during CNS development in mice.}, address = {Developmental Biology Laboratory, Maisonneuve-Rosemont Hospital, 5415 Boul. l'Assomption, Montreal, Canada, H1T 2M4.}, author = {Duparc, R. H. and Boutemmine, D. and Champagne, M. P. and T\'{e}treault, N. and Bernier, G. }, citeulike-article-id = {1361163}, doi = {10.1016/j.ydbio.2006.07.045}, issn = {0012-1606}, journal = {Dev Biol}, keywords = {d-catenin, pax6}, month = {December}, number = {2}, pages = {647--655}, posted-at = {2007-06-04 00:50:38}, priority = {2}, title = {Pax6 is required for delta-catenin/neurojugin expression during retinal, cerebellar and cortical development in mice.}, url = {http://dx.doi.org/10.1016/j.ydbio.2006.07.045}, volume = {300}, year = {2006} }

TY - JOUR ID - citeulike:1361163 L3 - citeulike-article-id:1361163 TI - Pax6 is required for delta-catenin/neurojugin expression during retinal, cerebellar and cortical development in mice. JF - Dev Biol VL - 300 IS - 2 SP - 647 EP - 655 AD - Developmental Biology Laboratory, Maisonneuve-Rosemont Hospital, 5415 Boul. l'Assomption, Montreal, Canada, H1T 2M4. SN - 0012-1606 N2 - The transcription factor Pax6 regulates multiple aspects of central nervous system (CNS) development. At the cellular level, the Pax6 mutation was reported to affect homophilic and heterophilic cellular adhesion, neuron polarity and neurite outgrowth. These abnormalities were observed in multiple regions of Pax6-mutant CNS, suggesting a common function for Pax6 in regulating cytoskeletal dynamics. However, target genes mediating Pax6 function in cytoskeletal dynamics remain largely unknown. Using DNA microarrays, we identified delta-catenin (delta-catenin /neurojugin) as a potential direct target of Pax6 in the CNS. delta-catenin encodes a large cytoskeletal protein that localizes at adherens junction in the CNS and that can modulate neurite outgrowth and N-cadherin turnover. delta-catenin was found to be co-expressed with Pax6 in several regions of the developing CNS. In Pax6 mutant embryos, delta-catenin expression was severely reduced in the optic vesicle neural ectoderm, in the ventricular zone of the neocortex and in the external granule layer of the cerebellum. We identified a Pax6 binding site in delta-catenin promoter that is conserved between mice and humans and which is effectively bound by Pax6 in vitro. Our results suggest that Pax6 regulates delta-catenin expression during CNS development in mice. KW - d-catenin KW - pax6 AU - Duparc, RH AU - Boutemmine, D AU - Champagne, MP AU - Tétreault, N AU - Bernier, G PY - 2006/12/15/ UR - http://dx.doi.org/10.1016/j.ydbio.2006.07.045 ER -