A new enzymatic method for determination of serum choline-containing phospholipids.

by: M Takayama, S Itoh, T Nagasaki, I Tanimizu

Clin Chim Acta, Vol. 79, No. 1. (15 August 1977), pp. 93-98.

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Abstract

A new enzymatic method is presented for the determination of serum choline-containing phospholipids with a combined enzymatic method using phospholipase D (from Streptomyces species), choline oxidase (from Arthrobacter species) and peroxidase. The method is reproducible, and the results correlate well with those obtained by the conventional digestion method (Hoeflmayr, J. and Fried, R. (1966) Med. Ernaehr. 7, 9-10). The method affords better specificity, requires a smaller quantity of the sample and shorter time than those previously reported, and has excellent precision.

@article{citeulike:100099, abstract = {A new enzymatic method is presented for the determination of serum choline-containing phospholipids with a combined enzymatic method using phospholipase D (from Streptomyces species), choline oxidase (from Arthrobacter species) and peroxidase. The method is reproducible, and the results correlate well with those obtained by the conventional digestion method (Hoeflmayr, J. and Fried, R. (1966) Med. Ernaehr. 7, 9-10). The method affords better specificity, requires a smaller quantity of the sample and shorter time than those previously reported, and has excellent precision.}, author = {Takayama, M. and Itoh, S. and Nagasaki, T. and Tanimizu, I. }, citeulike-article-id = {100099}, issn = {0009-8981}, journal = {Clin Chim Acta}, month = {August}, number = {1}, pages = {93--98}, posted-at = {2005-02-21 16:55:52}, priority = {2}, title = {A new enzymatic method for determination of serum choline-containing phospholipids.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/890967}, volume = {79}, year = {1977} }

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TY - JOUR ID - citeulike:100099 L3 - citeulike-article-id:100099 TI - A new enzymatic method for determination of serum choline-containing phospholipids. JF - Clin Chim Acta VL - 79 IS - 1 SP - 93 EP - 98 SN - 0009-8981 N2 - A new enzymatic method is presented for the determination of serum choline-containing phospholipids with a combined enzymatic method using phospholipase D (from Streptomyces species), choline oxidase (from Arthrobacter species) and peroxidase. The method is reproducible, and the results correlate well with those obtained by the conventional digestion method (Hoeflmayr, J. and Fried, R. (1966) Med. Ernaehr. 7, 9-10). The method affords better specificity, requires a smaller quantity of the sample and shorter time than those previously reported, and has excellent precision. AU - Takayama, M AU - Itoh, S AU - Nagasaki, T AU - Tanimizu, I PY - 1977/08/15/ UR - http://view.ncbi.nlm.nih.gov/pubmed/890967 ER -

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