NKG2D triggers cytotoxicity in mouse NK cells lacking DAP12 or Syk family kinases.

@article{citeulike:1441849, abstract = {In activated mouse natural killer (NK) cells, the NKG2D receptor associates with two intracellular adaptors, DAP10 and DAP12, which trigger phosphatidyl inositol 3 kinase (PI3K) and Syk family protein tyrosine kinases, respectively. Here we show that cytotoxicity, but not cytokine production, is triggered by NKG2D in activated NK cells lacking either DAP12 or the Syk family members Syk and ZAP70. Inhibition of PI3K blocks this cytotoxicity, suggesting that the DAP10-PI3K pathway is sufficient to initiate NKG2D-mediated killing of target cells. Our results highlight signaling divergence in the effector functions of NKG2D and indicate that alternative associations between a receptor and its adaptors may provide a single receptor with a dual 'on-switch', giving mouse NK cells more choices through which to trigger cytotoxicity.}, address = {Department of Immunology, Pasteur Institute, 25-28 rue Dr Roux, 75015 Paris, France.}, author = {Zompi, S. and Hamerman, J. A. and Ogasawara, K. and Schweighoffer, E. and Tybulewicz, V. L. and Di Santo, J. P. and Lanier, L. L. and Colucci, F. }, citeulike-article-id = {1441849}, doi = {http://dx.doi.org/10.1038/ni930}, issn = {1529-2908}, journal = {Nat Immunol}, keywords = {cytotoxic, dap10, dap12, deficient, ko, mouse, nkg2d, signaling, syk}, month = {June}, number = {6}, pages = {565--572}, posted-at = {2007-07-07 20:18:06}, priority = {3}, title = {NKG2D triggers cytotoxicity in mouse NK cells lacking DAP12 or Syk family kinases.}, url = {http://dx.doi.org/10.1038/ni930}, volume = {4}, year = {2003} }

TY - JOUR ID - citeulike:1441849 L3 - citeulike-article-id:1441849 TI - NKG2D triggers cytotoxicity in mouse NK cells lacking DAP12 or Syk family kinases. JF - Nat Immunol VL - 4 IS - 6 SP - 565 EP - 572 AD - Department of Immunology, Pasteur Institute, 25-28 rue Dr Roux, 75015 Paris, France. SN - 1529-2908 N2 - In activated mouse natural killer (NK) cells, the NKG2D receptor associates with two intracellular adaptors, DAP10 and DAP12, which trigger phosphatidyl inositol 3 kinase (PI3K) and Syk family protein tyrosine kinases, respectively. Here we show that cytotoxicity, but not cytokine production, is triggered by NKG2D in activated NK cells lacking either DAP12 or the Syk family members Syk and ZAP70. Inhibition of PI3K blocks this cytotoxicity, suggesting that the DAP10-PI3K pathway is sufficient to initiate NKG2D-mediated killing of target cells. Our results highlight signaling divergence in the effector functions of NKG2D and indicate that alternative associations between a receptor and its adaptors may provide a single receptor with a dual 'on-switch', giving mouse NK cells more choices through which to trigger cytotoxicity. KW - cytotoxic KW - dap10 KW - dap12 KW - deficient KW - ko KW - mouse KW - nkg2d KW - signaling KW - syk AU - Zompi, S AU - Hamerman, JA AU - Ogasawara, K AU - Schweighoffer, E AU - Tybulewicz, VL AU - Di Santo, JP AU - Lanier, LL AU - Colucci, F PY - 2003/06// UR - http://dx.doi.org/10.1038/ni930 ER -