Comparison of doxycycline delivery methods for Tet-inducible gene expression in a subcutaneous xenograft model.

@article{citeulike:1446266, abstract = {Doxycycline (Dox) controlled Tet systems provide a powerful and commonly used method for functional studies on the consequences of gene overexpression/downregulation. However, whereas Dox delivery in tissue culture in vitro is relatively simple, the situation in vivo is more complex. Several methods of Dox delivery in vivo have been described-e.g., in drinking water containing alcohol, in drinking water containing various concentrations of sucrose, and in feed. Unfortunately there are no reports directly comparing the advantages and disadvantages of these diverse methods, and there is no generally accepted standard. We therefore compared four non-invasive methods of Dox delivery in vivo-in drinking water, by gavage, as a jelly, and in standard feed. To assess the delivery of Dox by these methods, we used a subcutaneous xenograft model based on colorectal carcinoma cells engineered for Dox-inducible expression of an activated mutant of c-Src and the luciferase reporter gene. Our results indicate that feed represents the most favorable method of Dox administration.}, address = {Cancer Research UK, Paterson Institute for Cancer Research, University of Manchester, Manchester, UK.}, author = {Cawthorne, C. and Swindell, R. and Stratford, I. J. and Dive, C. and Welman, A. }, citeulike-article-id = {1446266}, issn = {1524-0215}, journal = {J Biomol Tech}, keywords = {delivery, dox, gedruckt, subcutaneous, tet, tumor, water}, month = {April}, number = {2}, pages = {120--123}, posted-at = {2007-07-10 11:43:07}, priority = {5}, title = {Comparison of doxycycline delivery methods for Tet-inducible gene expression in a subcutaneous xenograft model.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/17496224}, volume = {18}, year = {2007} }

TY - JOUR ID - citeulike:1446266 L3 - citeulike-article-id:1446266 TI - Comparison of doxycycline delivery methods for Tet-inducible gene expression in a subcutaneous xenograft model. JF - J Biomol Tech VL - 18 IS - 2 SP - 120 EP - 123 AD - Cancer Research UK, Paterson Institute for Cancer Research, University of Manchester, Manchester, UK. SN - 1524-0215 N2 - Doxycycline (Dox) controlled Tet systems provide a powerful and commonly used method for functional studies on the consequences of gene overexpression/downregulation. However, whereas Dox delivery in tissue culture in vitro is relatively simple, the situation in vivo is more complex. Several methods of Dox delivery in vivo have been described-e.g., in drinking water containing alcohol, in drinking water containing various concentrations of sucrose, and in feed. Unfortunately there are no reports directly comparing the advantages and disadvantages of these diverse methods, and there is no generally accepted standard. We therefore compared four non-invasive methods of Dox delivery in vivo-in drinking water, by gavage, as a jelly, and in standard feed. To assess the delivery of Dox by these methods, we used a subcutaneous xenograft model based on colorectal carcinoma cells engineered for Dox-inducible expression of an activated mutant of c-Src and the luciferase reporter gene. Our results indicate that feed represents the most favorable method of Dox administration. KW - delivery KW - dox KW - gedruckt KW - subcutaneous KW - tet KW - tumor KW - water AU - Cawthorne, C AU - Swindell, R AU - Stratford, IJ AU - Dive, C AU - Welman, A PY - 2007/04// UR - http://view.ncbi.nlm.nih.gov/pubmed/17496224 ER -