Immunogenicity and engraftment of mouse embryonic stem cells in allogeneic recipients.

@article{citeulike:1485420, abstract = {Embryonic stem cells (ESCs) are pluripotent and therefore able to differentiate both in vitro and in vivo into specialized tissues under appropriate conditions, a property that could be exploited for cellular therapies. However, the immunological nature of these cells in vivo has not been well understood. In vitro, mouse-derived ESCs fail to stimulate T cells, but they abrogate ongoing alloresponses by a process that requires cell-cell contact. We further show that despite a high expression of the NKG2D ligand retinoic acid early inducible-1 by mouse ESCs, they remain resistant to natural killer cell lysis. In vivo, allogeneic mouse ESCs populate the thymus, spleen, and liver of sublethally irradiated allogeneic host mice, inducing apoptosis to T cells and establishing multilineage mixed chimerism that significantly inhibits alloresponses to donor major histocompatibility complex antigens. Immunohistochemical imaging revealed a significant percentage of ESC-derived cells in the splenic marginal zones, but not in the follicles. Taken together, the data presented here reveal that nondifferentiated mouse embryonic stem cells are non-immunogenic and appear to populate lymphoid tissues in vivo, leading to T-cell deletion by apoptosis.}, address = {Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA.}, author = {Bonde, S. and Zavazava, N. }, citeulike-article-id = {1485420}, doi = {10.1634/stemcells.2006-0022}, issn = {1066-5099}, journal = {Stem Cells}, keywords = {cells, ligand, nkg2d, stem}, month = {October}, number = {10}, pages = {2192--2201}, posted-at = {2007-07-25 10:44:38}, priority = {2}, title = {Immunogenicity and engraftment of mouse embryonic stem cells in allogeneic recipients.}, url = {http://dx.doi.org/10.1634/stemcells.2006-0022}, volume = {24}, year = {2006} }

TY - JOUR ID - citeulike:1485420 L3 - citeulike-article-id:1485420 TI - Immunogenicity and engraftment of mouse embryonic stem cells in allogeneic recipients. JF - Stem Cells VL - 24 IS - 10 SP - 2192 EP - 2201 AD - Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA. SN - 1066-5099 N2 - Embryonic stem cells (ESCs) are pluripotent and therefore able to differentiate both in vitro and in vivo into specialized tissues under appropriate conditions, a property that could be exploited for cellular therapies. However, the immunological nature of these cells in vivo has not been well understood. In vitro, mouse-derived ESCs fail to stimulate T cells, but they abrogate ongoing alloresponses by a process that requires cell-cell contact. We further show that despite a high expression of the NKG2D ligand retinoic acid early inducible-1 by mouse ESCs, they remain resistant to natural killer cell lysis. In vivo, allogeneic mouse ESCs populate the thymus, spleen, and liver of sublethally irradiated allogeneic host mice, inducing apoptosis to T cells and establishing multilineage mixed chimerism that significantly inhibits alloresponses to donor major histocompatibility complex antigens. Immunohistochemical imaging revealed a significant percentage of ESC-derived cells in the splenic marginal zones, but not in the follicles. Taken together, the data presented here reveal that nondifferentiated mouse embryonic stem cells are non-immunogenic and appear to populate lymphoid tissues in vivo, leading to T-cell deletion by apoptosis. KW - cells KW - ligand KW - nkg2d KW - stem AU - Bonde, S AU - Zavazava, N PY - 2006/10// UR - http://dx.doi.org/10.1634/stemcells.2006-0022 ER -