Alignment of two-dimensional electrophoresis gels.

@article{citeulike:3038789, abstract = {Two-dimensional electrophoresis is a major separating technique for proteins in proteomics. Alignment of gel images is critical for intra-laboratory or even more difficult inter-laboratory gel comparisons. In the paper, we propose a novel iterative closest point (ICP) method for 2D-gel electrophoresis image alignment. The paper seeks to introduce an information theoretic measure as one part of distance metric to gel image alignment. We combine intensity information of spots with geometric information of landmarks by applying information potential idea. The proposed method has been applied to both synthetic and real gel images accessible in public 2D-electrophoresis gel protein databases. The high accuracy and robustness of the algorithm indicate that it is promising for gel image alignment.}, address = {National Laboratory of Pattern Recognition, Institute of Automation, Chinese Academy of Sciences, Beijing 100080, PR China.}, author = {Shi, G. and Jiang, T. and Zhu, W. and Liu, B. and Zhao, H. }, citeulike-article-id = {3038789}, doi = {http://dx.doi.org/10.1016/j.bbrc.2007.03.165}, issn = {0006-291X}, journal = {Biochemical and biophysical research communications}, keywords = {2dge}, month = {June}, number = {2}, pages = {427--432}, posted-at = {2008-07-24 10:22:55}, priority = {2}, title = {Alignment of two-dimensional electrophoresis gels.}, url = {http://dx.doi.org/10.1016/j.bbrc.2007.03.165}, volume = {357}, year = {2007} }

TY - JOUR ID - citeulike:3038789 L3 - citeulike-article-id:3038789 TI - Alignment of two-dimensional electrophoresis gels. JF - Biochemical and biophysical research communications VL - 357 IS - 2 SP - 427 EP - 432 AD - National Laboratory of Pattern Recognition, Institute of Automation, Chinese Academy of Sciences, Beijing 100080, PR China. SN - 0006-291X N2 - Two-dimensional electrophoresis is a major separating technique for proteins in proteomics. Alignment of gel images is critical for intra-laboratory or even more difficult inter-laboratory gel comparisons. In the paper, we propose a novel iterative closest point (ICP) method for 2D-gel electrophoresis image alignment. The paper seeks to introduce an information theoretic measure as one part of distance metric to gel image alignment. We combine intensity information of spots with geometric information of landmarks by applying information potential idea. The proposed method has been applied to both synthetic and real gel images accessible in public 2D-electrophoresis gel protein databases. The high accuracy and robustness of the algorithm indicate that it is promising for gel image alignment. KW - 2dge AU - Shi, G AU - Jiang, T AU - Zhu, W AU - Liu, B AU - Zhao, H PY - 2007/06/01/ UR - http://dx.doi.org/10.1016/j.bbrc.2007.03.165 ER -