Dynamic reassembly of peptide RADA16 nanofiber scaffold

@article{citeulike:970575, abstract = {Nanofiber structures of some peptides and proteins as biological materials have been studied extensively, but their molecular mechanism of self-assembly and reassembly still remains unclear. We report here the reassembly of an ionic self-complementary peptide RADARADARADARADA (RADA16-I) that forms a well defined nanofiber scaffold. The 16-residue peptide forms stable {beta}-sheet structure and undergoes molecular self-assembly into nanofibers and eventually a scaffold hydrogel consisting of >99.5\% water. In this study, the nanofiber scaffold was sonicated into smaller fragments. Circular dichroism, atomic force microscopy, and rheology were used to follow the kinetics of the reassembly. These sonicated fragments not only quickly reassemble into nanofibers that were indistinguishable from the original material, but their reassembly also correlated with the rheological analyses showing an increase of scaffold rigidity as a function of nanofiber length. The disassembly and reassembly processes were repeated four times and, each time, the reassembly reached the original length. We proposed a plausible sliding diffusion model to interpret the reassembly involving complementary nanofiber cohesive ends. This reassembly process is important for fabrication of new scaffolds for 3D cell culture, tissue repair, and regenerative medicine. 10.1073/pnas.0407843102}, author = {Yokoi, Hidenori and Kinoshita, Takatoshi and Zhang, Shuguang }, citeulike-article-id = {970575}, journal = {PNAS}, month = {June}, number = {24}, pages = {8414--8419}, posted-at = {2006-12-01 16:24:48}, priority = {2}, title = {Dynamic reassembly of peptide RADA16 nanofiber scaffold}, url = {http://www.pnas.org/cgi/content/abstract/102/24/8414}, volume = {102}, year = {2005} }

TY - JOUR ID - citeulike:970575 L3 - citeulike-article-id:970575 TI - Dynamic reassembly of peptide RADA16 nanofiber scaffold JF - PNAS VL - 102 IS - 24 SP - 8414 EP - 8419 N2 - Nanofiber structures of some peptides and proteins as biological materials have been studied extensively, but their molecular mechanism of self-assembly and reassembly still remains unclear. We report here the reassembly of an ionic self-complementary peptide RADARADARADARADA (RADA16-I) that forms a well defined nanofiber scaffold. The 16-residue peptide forms stable {beta}-sheet structure and undergoes molecular self-assembly into nanofibers and eventually a scaffold hydrogel consisting of >99.5% water. In this study, the nanofiber scaffold was sonicated into smaller fragments. Circular dichroism, atomic force microscopy, and rheology were used to follow the kinetics of the reassembly. These sonicated fragments not only quickly reassemble into nanofibers that were indistinguishable from the original material, but their reassembly also correlated with the rheological analyses showing an increase of scaffold rigidity as a function of nanofiber length. The disassembly and reassembly processes were repeated four times and, each time, the reassembly reached the original length. We proposed a plausible sliding diffusion model to interpret the reassembly involving complementary nanofiber cohesive ends. This reassembly process is important for fabrication of new scaffolds for 3D cell culture, tissue repair, and regenerative medicine. 10.1073/pnas.0407843102 AU - Yokoi, Hidenori AU - Kinoshita, Takatoshi AU - Zhang, Shuguang PY - 2005/06/14/ UR - http://www.pnas.org/cgi/content/abstract/102/24/8414 ER -