Influence of the shape of phospholipid vesicles on the measurement of their size by photon correlation spectroscopy.

@article{citeulike:1567374, abstract = {The influence of shape transformation of large unilamellar vesicles (LUV) on their size measurement by photon correlation spectroscopy (PCS) has been investigated. The experimental size of vesicles after hyperosmotic contractions of increasing intensities have been compared to the theoretical volume decrease determined by applying Boyle Van't Hoff's law. The main observation is that PCS size measurement gives overestimated values when LUV have been subjected to a volume decrease of more than 20\% of their initial volume. The PCS size overestimation is related to the influence of the shape transformation of the vesicles on their diffusion coefficient (D) as shown by modelling the evolution of D of a sphere which is transformed into an ellipsoid by internal volume reduction under constant area.}, address = {Laboratoire de G\'{e}nie des Proc\'{e}d\'{e}s Alimentaires et Biotechnologiques, Ecole Nationale Sup\'{e}rieure de Biologie Appliqu\'{e}e \`{a} l'Alimentation, Dijon, France.}, author = {Beney, L. and Linares, E. and Ferret, E. and Gervais, P. }, citeulike-article-id = {1567374}, issn = {0175-7571}, journal = {Eur Biophys J}, keywords = {correlation, pcs, phospholipid, photon, shape, spectroscopy, vesicles}, number = {6}, pages = {567--574}, posted-at = {2007-08-15 17:05:11}, priority = {2}, title = {Influence of the shape of phospholipid vesicles on the measurement of their size by photon correlation spectroscopy.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/9791939}, volume = {27}, year = {1998} }

TY - JOUR ID - citeulike:1567374 L3 - citeulike-article-id:1567374 TI - Influence of the shape of phospholipid vesicles on the measurement of their size by photon correlation spectroscopy. JF - Eur Biophys J VL - 27 IS - 6 SP - 567 EP - 574 AD - Laboratoire de Génie des Procédés Alimentaires et Biotechnologiques, Ecole Nationale Supérieure de Biologie Appliquée à l'Alimentation, Dijon, France. SN - 0175-7571 N2 - The influence of shape transformation of large unilamellar vesicles (LUV) on their size measurement by photon correlation spectroscopy (PCS) has been investigated. The experimental size of vesicles after hyperosmotic contractions of increasing intensities have been compared to the theoretical volume decrease determined by applying Boyle Van't Hoff's law. The main observation is that PCS size measurement gives overestimated values when LUV have been subjected to a volume decrease of more than 20% of their initial volume. The PCS size overestimation is related to the influence of the shape transformation of the vesicles on their diffusion coefficient (D) as shown by modelling the evolution of D of a sphere which is transformed into an ellipsoid by internal volume reduction under constant area. KW - correlation KW - pcs KW - phospholipid KW - photon KW - shape KW - spectroscopy KW - vesicles AU - Beney, L AU - Linares, E AU - Ferret, E AU - Gervais, P PY - 1998/// UR - http://view.ncbi.nlm.nih.gov/pubmed/9791939 ER -