Synthesis and Characterization of Fluorescently Doped Mesoporous Nanoparticles for Two-Photon Excitation

@article{citeulike:2793329, abstract = {Abstract: The template-directed synthesis of mesoporous silica nanoparticles doped with water-soluble fluorescent dyes optimized for two-photon excitation is described. Two structurally related symmetrical two-photon dyes that possess pyridinium acceptor endgroups conjugated to a fluorenyl core are synthesized by Heck coupling. These dyes display bright fluorescence (¦ H 0.35) under both one- (µ H 6 × 104 M1 cm1) and two-photon excitation (\~{A}2 H 1000 GM) and were successfully encapsulated in silica nanoparticles via immobilization through noncovalent interactions. The nanoparticles present a mean diameter of 100 nm and a hexagonal network of mesopores as shown by TEM. Interestingly, the photophysical characteristics of the dyes are retained upon their immobilization into the silica matrix, leading to fluorescent silica nanoparticles with giant/unprecedented TPA cross-section (1 × 107 GM). Such nanospheres represent attractive nanoplatforms for the development of biotargeted biocompatible luminescent tracers.}, address = {Universit\'{e} Montpellier 2, ENSCM, Institut Charles Gerhardt Montpellier, CNRS, UMR 5253, CC1701 Place Eug\`{e}ne Bataillon, 34095 Montpellier Cedex 05, France, Universit\'{e} Montpellier 2, Institut Europ\'{e}en des Membranes, CNRS, UMR 5635, 1919 Route de Mende, 34293 Montpellier Cedex 05, France, and Universit\'{e} Rennes 1, Institut de Chimie, CNRS, UMR 6510, Campus de Beaulieu, 35042 Rennes Cedex, France}, author = {Lebret, Val\&\#xe9;rie and Raehm, Laurence and Durand, Jean-Olivier and Sma\&\#xef;hi, Monique and G\&\#xe9;rardin, Corine and Nerambourg, Nicolas and Werts, Martinus H. and Blanchard-Desce, Mireille }, citeulike-article-id = {2793329}, doi = {http://dx.doi.org/10.1021/cm703487b}, journal = {Chem. Mater.}, keywords = {characterization, citedby, excitation, fluorescence, mesoporous, nanoparticles, synthesis, two-photon}, month = {March}, number = {6}, pages = {2174--2183}, posted-at = {2008-05-13 04:59:43}, priority = {2}, title = {Synthesis and Characterization of Fluorescently Doped Mesoporous Nanoparticles for Two-Photon Excitation}, url = {http://dx.doi.org/10.1021/cm703487b}, volume = {20}, year = {2008} }

TY - JOUR ID - citeulike:2793329 L3 - citeulike-article-id:2793329 TI - Synthesis and Characterization of Fluorescently Doped Mesoporous Nanoparticles for Two-Photon Excitation JF - Chem. Mater. VL - 20 IS - 6 SP - 2174 EP - 2183 AD - Université Montpellier 2, ENSCM, Institut Charles Gerhardt Montpellier, CNRS, UMR 5253, CC1701 Place Eugène Bataillon, 34095 Montpellier Cedex 05, France, Université Montpellier 2, Institut Européen des Membranes, CNRS, UMR 5635, 1919 Route de Mende, 34293 Montpellier Cedex 05, France, and Université Rennes 1, Institut de Chimie, CNRS, UMR 6510, Campus de Beaulieu, 35042 Rennes Cedex, France N2 - Abstract: The template-directed synthesis of mesoporous silica nanoparticles doped with water-soluble fluorescent dyes optimized for two-photon excitation is described. Two structurally related symmetrical two-photon dyes that possess pyridinium acceptor endgroups conjugated to a fluorenyl core are synthesized by Heck coupling. These dyes display bright fluorescence (¦ H 0.35) under both one- (µ H 6 × 104 M1 cm1) and two-photon excitation (Ã2 H 1000 GM) and were successfully encapsulated in silica nanoparticles via immobilization through noncovalent interactions. The nanoparticles present a mean diameter of 100 nm and a hexagonal network of mesopores as shown by TEM. Interestingly, the photophysical characteristics of the dyes are retained upon their immobilization into the silica matrix, leading to fluorescent silica nanoparticles with giant/unprecedented TPA cross-section (1 × 107 GM). Such nanospheres represent attractive nanoplatforms for the development of biotargeted biocompatible luminescent tracers. KW - characterization KW - citedby KW - excitation KW - fluorescence KW - mesoporous KW - nanoparticles KW - synthesis KW - two-photon AU - Lebret, Valérie AU - Raehm, Laurence AU - Durand, Jean-Olivier AU - Smaïhi, Monique AU - Gérardin, Corine AU - Nerambourg, Nicolas AU - Werts, Martinus AU - Blanchard-Desce, Mireille PY - 2008/03/25/ UR - http://dx.doi.org/10.1021/cm703487b ER -