Convective-Diffusion-Based Fluorescence Correlation Spectroscopy for Detection of a Trace Amount of E. coli in Water

by: De-Kui Qing, Pinar M Mengüç, Fred A Payne, Mary-Grace C Danao

Appl. Opt., Vol. 42, No. 16. (1 June 2003), pp. 2987-2994.

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Optical Society of America

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Abstract

Fluorescence correlation spectroscopy (FCS) is adapted for a new procedure to detect trace amounts of Escherichia coli in water. The present concept is based on convective diffusion rather than Brownian diffusion and employs confocal microscopy as in traditional FCS. With this system it is possible to detect concentrations as small as 1.5 × 10 5 E. coli per milliliter (2.5 × 10 −16 M). This concentration corresponds to an ~1.0-nM level of Rhodamine 6G dyes. A detailed analysis of the optical system is presented, and further improvements for the procedure are discussed.

@article{citeulike:2853472, abstract = {Fluorescence correlation spectroscopy (FCS) is adapted for a new procedure to detect trace amounts of Escherichia coli in water. The present concept is based on convective diffusion rather than Brownian diffusion and employs confocal microscopy as in traditional FCS. With this system it is possible to detect concentrations as small as 1.5 × 10 5 E. coli per milliliter (2.5 × 10 −16 M). This concentration corresponds to an \~{}1.0-nM level of Rhodamine 6G dyes. A detailed analysis of the optical system is presented, and further improvements for the procedure are discussed.}, author = {Qing, De-Kui and Meng\"{u}ç, Pinar M. and Payne, Fred A. and Danao, Mary-Grace C. }, citeulike-article-id = {2853472}, journal = {Appl. Opt.}, keywords = {coli, correlation, detection, diffusion, ecoli, escherichia, fcs, fluorescence, spectroscopy, water}, month = {June}, number = {16}, pages = {2987--2994}, posted-at = {2008-05-31 18:27:36}, priority = {2}, publisher = {OSA}, title = {Convective-Diffusion-Based Fluorescence Correlation Spectroscopy for Detection of a Trace Amount of E. coli in Water}, url = {http://www.opticsinfobase.org/abstract.cfm?id=72455}, volume = {42}, year = {2003} }

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TY - JOUR ID - citeulike:2853472 L3 - citeulike-article-id:2853472 TI - Convective-Diffusion-Based Fluorescence Correlation Spectroscopy for Detection of a Trace Amount of E. coli in Water JF - Appl. Opt. VL - 42 IS - 16 SP - 2987 EP - 2994 PB - OSA N2 - Fluorescence correlation spectroscopy (FCS) is adapted for a new procedure to detect trace amounts of Escherichia coli in water. The present concept is based on convective diffusion rather than Brownian diffusion and employs confocal microscopy as in traditional FCS. With this system it is possible to detect concentrations as small as 1.5 × 10 5 E. coli per milliliter (2.5 × 10 −16 M). This concentration corresponds to an ~1.0-nM level of Rhodamine 6G dyes. A detailed analysis of the optical system is presented, and further improvements for the procedure are discussed. KW - coli KW - correlation KW - detection KW - diffusion KW - ecoli KW - escherichia KW - fcs KW - fluorescence KW - spectroscopy KW - water AU - Qing, De-Kui AU - Mengüç, Pinar AU - Payne, Fred AU - Danao, Mary-Grace PY - 2003/06/01/ UR - http://www.opticsinfobase.org/abstract.cfm?id=72455 ER -

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