Absorption Coefficient Imaging by Near-Field Scanning Optical Microscopy in Bacteria

by: Ana M de Paula, Claudilene R Chaves, Haroldo B Silva, Gerald Weber

Appl. Opt., Vol. 42, No. 16. (1 June 2003), pp. 3005-3008.

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Optical Society of America

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Abstract

We present a method for obtaining a position-dependent absorption coefficient from near-field scanning optical transmission microscopy. We show that the optical transmission intensity can be combined with the topography, resulting into an absorption coefficient that simplifies the analysis of different materials within a sample. The method is tested with the dye rhodamine 6G, and we show some analysis in biological samples such as bacteria Klebsiella pneumoniae and Pseudomonas aeruginosa . The calculated absorption coefficient images show important details of the bacteria, in particular for P. aeruginosa , in which membrane vesicles are clearly seen.

@article{citeulike:2853476, abstract = {We present a method for obtaining a position-dependent absorption coefficient from near-field scanning optical transmission microscopy. We show that the optical transmission intensity can be combined with the topography, resulting into an absorption coefficient that simplifies the analysis of different materials within a sample. The method is tested with the dye rhodamine 6G, and we show some analysis in biological samples such as bacteria Klebsiella pneumoniae and Pseudomonas aeruginosa . The calculated absorption coefficient images show important details of the bacteria, in particular for P. aeruginosa , in which membrane vesicles are clearly seen.}, author = {de Paula, Ana M. and Chaves, Claudilene R. and Silva, Haroldo B. and Weber, Gerald }, citeulike-article-id = {2853476}, journal = {Appl. Opt.}, keywords = {absorption, bacteria, imaging, microscopy, near-field, nsom, optical, scanning}, month = {June}, number = {16}, pages = {3005--3008}, posted-at = {2008-05-31 18:30:01}, priority = {2}, publisher = {OSA}, title = {Absorption Coefficient Imaging by Near-Field Scanning Optical Microscopy in Bacteria}, url = {http://www.opticsinfobase.org/abstract.cfm?id=72457}, volume = {42}, year = {2003} }

RIS record

TY - JOUR ID - citeulike:2853476 L3 - citeulike-article-id:2853476 TI - Absorption Coefficient Imaging by Near-Field Scanning Optical Microscopy in Bacteria JF - Appl. Opt. VL - 42 IS - 16 SP - 3005 EP - 3008 PB - OSA N2 - We present a method for obtaining a position-dependent absorption coefficient from near-field scanning optical transmission microscopy. We show that the optical transmission intensity can be combined with the topography, resulting into an absorption coefficient that simplifies the analysis of different materials within a sample. The method is tested with the dye rhodamine 6G, and we show some analysis in biological samples such as bacteria Klebsiella pneumoniae and Pseudomonas aeruginosa . The calculated absorption coefficient images show important details of the bacteria, in particular for P. aeruginosa , in which membrane vesicles are clearly seen. KW - absorption KW - bacteria KW - imaging KW - microscopy KW - near-field KW - nsom KW - optical KW - scanning AU - de Paula, Ana AU - Chaves, Claudilene AU - Silva, Haroldo AU - Weber, Gerald PY - 2003/06/01/ UR - http://www.opticsinfobase.org/abstract.cfm?id=72457 ER -

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