Plasma membrane-associated proteins are clustered into islands attached to the cytoskeleton.

@article{citeulike:992587, abstract = {Although much evidence suggests that the plasma membrane of eukaryotic cells is not homogenous, the precise architecture of this important structure has not been clear. Here we use transmission electron microscopy of plasma membrane sheets and specific probes to show that most or all plasma membrane-associated proteins are clustered in cholesterol-enriched domains ("islands") that are separated by "protein-free" and cholesterol-low membrane. These islands are further divided into subregions, as shown by the localization of "raft" and "non-raft" markers to specific areas. Abundant actin staining and inhibitor studies show that these structures are connected to the cytoskeleton and at least partially depend on it for their formation and/or maintenance.}, address = {Howard Hughes Medical Institute and Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305.}, author = {Lillemeier, Bj\"{o}rn F F. and Pfeiffer, Janet R R. and Surviladze, Zurab and Wilson, Bridget S S. and Davis, Mark M M. }, citeulike-article-id = {992587}, doi = {10.1073/pnas.0609009103}, issn = {0027-8424}, journal = {Proc Natl Acad Sci U S A}, keywords = {domains, rafts}, month = {December}, posted-at = {2008-06-26 19:37:51}, priority = {2}, title = {Plasma membrane-associated proteins are clustered into islands attached to the cytoskeleton.}, url = {http://dx.doi.org/10.1073/pnas.0609009103}, year = {2006} }

TY - JOUR ID - citeulike:992587 L3 - citeulike-article-id:992587 TI - Plasma membrane-associated proteins are clustered into islands attached to the cytoskeleton. JF - Proc Natl Acad Sci U S A AD - Howard Hughes Medical Institute and Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305. SN - 0027-8424 N2 - Although much evidence suggests that the plasma membrane of eukaryotic cells is not homogenous, the precise architecture of this important structure has not been clear. Here we use transmission electron microscopy of plasma membrane sheets and specific probes to show that most or all plasma membrane-associated proteins are clustered in cholesterol-enriched domains ("islands") that are separated by "protein-free" and cholesterol-low membrane. These islands are further divided into subregions, as shown by the localization of "raft" and "non-raft" markers to specific areas. Abundant actin staining and inhibitor studies show that these structures are connected to the cytoskeleton and at least partially depend on it for their formation and/or maintenance. KW - domains KW - rafts AU - Lillemeier, Björn F AU - Pfeiffer, Janet R AU - Surviladze, Zurab AU - Wilson, Bridget S AU - Davis, Mark M PY - 2006/12/04/ UR - http://dx.doi.org/10.1073/pnas.0609009103 ER -