The asexual yeast Candida glabrata maintains distinct a and alpha haploid mating types.

@article{citeulike:3023371, abstract = {The genome of the type strain of Candida glabrata (CBS138, ATCC 2001) contains homologs of most of the genes involved in mating in Saccharomyces cerevisiae, starting with the mating pheromone and receptor genes. Only haploid cells are ever isolated, but C. glabrata strains of both mating types are commonly found, the type strain being MAT alpha and most other strains, such as BG2, being MATa. No sexual cycle has been documented for this species. In order to understand which steps of the mating pathway are defective, we have analyzed the expression of homologs of some of the key genes involved as well as the production of mating pheromones and the organism's sensitivity to artificial pheromones. We show that cells of opposite mating types express both pheromone receptor genes and are insensitive to pheromones. Nonetheless, cells maintain specificity through regulation of the alpha1 and alpha2 genes and, more surprisingly, through differential splicing of the a1 transcript.}, address = {Institut Pasteur, Unit\'{e} de G\'{e}n\'{e}tique Mol\'{e}culaire des Levures, CNRS URA 2171, Universit\'{e} Pierre et Marie Curie-Paris 6 UFR927, F75015 Paris, France. hmuller@pasteur.fr}, author = {Muller, H. and Hennequin, C. and Gallaud, J. and Dujon, B. and Fairhead, C. }, citeulike-article-id = {3023371}, doi = {http://dx.doi.org/10.1128/EC.00456-07}, issn = {1535-9786}, journal = {Eukaryotic cell}, keywords = {candida, fungi, genome, mating\_genes, mating\_type, saccharomyces}, month = {May}, number = {5}, pages = {848--858}, posted-at = {2008-07-21 04:13:59}, priority = {2}, title = {The asexual yeast Candida glabrata maintains distinct a and alpha haploid mating types.}, url = {http://dx.doi.org/10.1128/EC.00456-07}, volume = {7}, year = {2008} }

TY - JOUR ID - citeulike:3023371 L3 - citeulike-article-id:3023371 TI - The asexual yeast Candida glabrata maintains distinct a and alpha haploid mating types. JF - Eukaryotic cell VL - 7 IS - 5 SP - 848 EP - 858 AD - Institut Pasteur, Unité de Génétique Moléculaire des Levures, CNRS URA 2171, Université Pierre et Marie Curie-Paris 6 UFR927, F75015 Paris, France. hmuller@pasteur.fr SN - 1535-9786 N2 - The genome of the type strain of Candida glabrata (CBS138, ATCC 2001) contains homologs of most of the genes involved in mating in Saccharomyces cerevisiae, starting with the mating pheromone and receptor genes. Only haploid cells are ever isolated, but C. glabrata strains of both mating types are commonly found, the type strain being MAT alpha and most other strains, such as BG2, being MATa. No sexual cycle has been documented for this species. In order to understand which steps of the mating pathway are defective, we have analyzed the expression of homologs of some of the key genes involved as well as the production of mating pheromones and the organism's sensitivity to artificial pheromones. We show that cells of opposite mating types express both pheromone receptor genes and are insensitive to pheromones. Nonetheless, cells maintain specificity through regulation of the alpha1 and alpha2 genes and, more surprisingly, through differential splicing of the a1 transcript. KW - candida KW - fungi KW - genome KW - mating_genes KW - mating_type KW - saccharomyces AU - Muller, H AU - Hennequin, C AU - Gallaud, J AU - Dujon, B AU - Fairhead, C PY - 2008/05// UR - http://dx.doi.org/10.1128/EC.00456-07 ER -