Role of cysteine in regulating morphogenesis and mitochondrial activity in the dimorphic fungus Histoplasma capsulatum.

@article{citeulike:652300, abstract = {Three stages can be distinguished in the temperature-induced mycelial-to-yeast phase transition of Histoplasma capsulatum. Stage one is characterized by a progressive decrease in the respiration rate and in the intracellular concentrations of cysteine and other amino acids. By stage two, respiration has ceased completely and free cysteine has fallen to low levels. Exogenous cysteine is required during the second stage for activation of mitochondrial respiration (stage three) and completion of the morphological transition. Mitochondria isolated from cells in the second stage show no respiration with NADH, succinate, or other substrates unless they are first incubated with cysteine. In addition, a novel, cytosolic cysteine oxidase appears during the latter part of the second stage. In stage three, the respiration rate rises, intracellular concentrations of free cysteine and other amino acids increase to levels characteristic of yeast, and the morphological transition is completed. The results support the idea that alterations in cysteine metabolism play a key role in this differentiation process.}, author = {Maresca, B. and Lambowitz, A. M. and Kumar, V. B. and Grant, G. A. and Kobayashi, G. S. and Medoff, G. }, citeulike-article-id = {652300}, issn = {0027-8424}, journal = {Proc Natl Acad Sci U S A}, keywords = {dimorphism, fungi, histoplasma, morphogenesis}, month = {July}, number = {7}, pages = {4596--4600}, posted-at = {2007-06-12 01:00:08}, priority = {2}, title = {Role of cysteine in regulating morphogenesis and mitochondrial activity in the dimorphic fungus Histoplasma capsulatum.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/6945601}, volume = {78}, year = {1981} }

TY - JOUR ID - citeulike:652300 L3 - citeulike-article-id:652300 TI - Role of cysteine in regulating morphogenesis and mitochondrial activity in the dimorphic fungus Histoplasma capsulatum. JF - Proc Natl Acad Sci U S A VL - 78 IS - 7 SP - 4596 EP - 4600 SN - 0027-8424 N2 - Three stages can be distinguished in the temperature-induced mycelial-to-yeast phase transition of Histoplasma capsulatum. Stage one is characterized by a progressive decrease in the respiration rate and in the intracellular concentrations of cysteine and other amino acids. By stage two, respiration has ceased completely and free cysteine has fallen to low levels. Exogenous cysteine is required during the second stage for activation of mitochondrial respiration (stage three) and completion of the morphological transition. Mitochondria isolated from cells in the second stage show no respiration with NADH, succinate, or other substrates unless they are first incubated with cysteine. In addition, a novel, cytosolic cysteine oxidase appears during the latter part of the second stage. In stage three, the respiration rate rises, intracellular concentrations of free cysteine and other amino acids increase to levels characteristic of yeast, and the morphological transition is completed. The results support the idea that alterations in cysteine metabolism play a key role in this differentiation process. KW - dimorphism KW - fungi KW - histoplasma KW - morphogenesis AU - Maresca, B AU - Lambowitz, AM AU - Kumar, VB AU - Grant, GA AU - Kobayashi, GS AU - Medoff, G PY - 1981/07// UR - http://view.ncbi.nlm.nih.gov/pubmed/6945601 ER -