Molecular Anatomy of a Trafficking Organelle.

@article{citeulike:955135, abstract = {Membrane traffic in eukaryotic cells involves transport of vesicles that bud from a donor compartment and fuse with an acceptor compartment. Common principles of budding and fusion have emerged, and many of the proteins involved in these events are now known. However, a detailed picture of an entire trafficking organelle is not yet available. Using synaptic vesicles as a model, we have now determined the protein and lipid composition; measured vesicle size, density, and mass; calculated the average protein and lipid mass per vesicle; and determined the copy number of more than a dozen major constituents. A model has been constructed that integrates all quantitative data and includes structural models of abundant proteins. Synaptic vesicles are dominated by proteins, possess a surprising diversity of trafficking proteins, and, with the exception of the V-ATPase that is present in only one to two copies, contain numerous copies of proteins essential for membrane traffic and neurotransmitter uptake.}, address = {Department of Neurobiology, Max-Planck Institute for Biophysical Chemistry, 37077 G\"{o}ttingen, Germany.}, author = {Takamori, Shigeo and Holt, Matthew and Stenius, Katinka and Lemke, Edward A A. and Grønborg, Mads and Riedel, Dietmar and Urlaub, Henning and Schenck, Stephan and Br\"{u}gger, Britta and Ringler, Philippe and M\"{u}ller, Shirley A A. and Rammner, Burkhard and Gr\"{a}ter, Frauke and Hub, Jochen S S. and De Groot, Bert L L. and Mieskes, Gottfried and Moriyama, Yoshinori and Klingauf, J\"{u}rgen and Grubm\"{u}ller, Helmut and Heuser, John and Wieland, Felix and Jahn, Reinhard }, citeulike-article-id = {955135}, doi = {http://dx.doi.org/10.1016/j.cell.2006.10.030}, issn = {0092-8674}, journal = {Cell}, keywords = {synaptic, vesicles}, month = {November}, number = {4}, pages = {831--846}, posted-at = {2007-01-03 22:01:20}, priority = {2}, title = {Molecular Anatomy of a Trafficking Organelle.}, url = {http://dx.doi.org/10.1016/j.cell.2006.10.030}, volume = {127}, year = {2006} }

TY - JOUR ID - citeulike:955135 L3 - citeulike-article-id:955135 TI - Molecular Anatomy of a Trafficking Organelle. JF - Cell VL - 127 IS - 4 SP - 831 EP - 846 AD - Department of Neurobiology, Max-Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany. SN - 0092-8674 N2 - Membrane traffic in eukaryotic cells involves transport of vesicles that bud from a donor compartment and fuse with an acceptor compartment. Common principles of budding and fusion have emerged, and many of the proteins involved in these events are now known. However, a detailed picture of an entire trafficking organelle is not yet available. Using synaptic vesicles as a model, we have now determined the protein and lipid composition; measured vesicle size, density, and mass; calculated the average protein and lipid mass per vesicle; and determined the copy number of more than a dozen major constituents. A model has been constructed that integrates all quantitative data and includes structural models of abundant proteins. Synaptic vesicles are dominated by proteins, possess a surprising diversity of trafficking proteins, and, with the exception of the V-ATPase that is present in only one to two copies, contain numerous copies of proteins essential for membrane traffic and neurotransmitter uptake. KW - synaptic KW - vesicles AU - Takamori, Shigeo AU - Holt, Matthew AU - Stenius, Katinka AU - Lemke, Edward A AU - Grønborg, Mads AU - Riedel, Dietmar AU - Urlaub, Henning AU - Schenck, Stephan AU - Brügger, Britta AU - Ringler, Philippe AU - Müller, Shirley A AU - Rammner, Burkhard AU - Gräter, Frauke AU - Hub, Jochen S AU - De Groot, Bert L AU - Mieskes, Gottfried AU - Moriyama, Yoshinori AU - Klingauf, Jürgen AU - Grubmüller, Helmut AU - Heuser, John AU - Wieland, Felix AU - Jahn, Reinhard PY - 2006/11/17/ UR - http://dx.doi.org/10.1016/j.cell.2006.10.030 ER -