A genome-scale map of expression for a mouse brain section obtained using voxelation.

@article{citeulike:3033244, abstract = {Gene expression signatures in the mammalian brain hold the key to understanding neural development and neurological diseases. We have reconstructed two-dimensional images of gene expression for 20,000 genes in a coronal slice of the mouse brain at the level of the striatum by using microarrays in combination with voxelation at a resolution of 1 mm3. Good reliability of the microarray results were confirmed using multiple replicates, subsequent quantitative RT-PCR voxelation, mass spectrometry voxelation, and publicly available in situ hybridization data. Known and novel genes were identified with expression patterns localized to defined substructures within the brain. In addition, genes with unexpected patterns were identified, and cluster analysis identified a set of genes with a gradient of dorsal/ventral expression not restricted to known anatomical boundaries. The genome-scale maps of gene expression obtained using voxelation will be a valuable tool for the neuroscience community.}, address = {Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, California, USA.}, author = {Chin, M. H. and Geng, A. B. and Khan, A. H. and Qian, W. J. and Petyuk, V. A. and Boline, J. and Levy, S. and Toga, A. W. and Smith, R. D. and Leahy, R. M. and Smith, D. J. }, citeulike-article-id = {3033244}, doi = {10.1152/physiolgenomics.00287.2006}, issn = {1531-2267}, journal = {Physiological genomics}, keywords = {vmsr\_publications}, month = {August}, number = {3}, pages = {313--321}, posted-at = {2008-07-22 18:49:19}, priority = {2}, title = {A genome-scale map of expression for a mouse brain section obtained using voxelation.}, url = {http://dx.doi.org/10.1152/physiolgenomics.00287.2006}, volume = {30}, year = {2007} }

TY - JOUR ID - citeulike:3033244 L3 - citeulike-article-id:3033244 TI - A genome-scale map of expression for a mouse brain section obtained using voxelation. JF - Physiological genomics VL - 30 IS - 3 SP - 313 EP - 321 AD - Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, California, USA. SN - 1531-2267 N2 - Gene expression signatures in the mammalian brain hold the key to understanding neural development and neurological diseases. We have reconstructed two-dimensional images of gene expression for 20,000 genes in a coronal slice of the mouse brain at the level of the striatum by using microarrays in combination with voxelation at a resolution of 1 mm3. Good reliability of the microarray results were confirmed using multiple replicates, subsequent quantitative RT-PCR voxelation, mass spectrometry voxelation, and publicly available in situ hybridization data. Known and novel genes were identified with expression patterns localized to defined substructures within the brain. In addition, genes with unexpected patterns were identified, and cluster analysis identified a set of genes with a gradient of dorsal/ventral expression not restricted to known anatomical boundaries. The genome-scale maps of gene expression obtained using voxelation will be a valuable tool for the neuroscience community. KW - vmsr_publications AU - Chin, MH AU - Geng, AB AU - Khan, AH AU - Qian, WJ AU - Petyuk, VA AU - Boline, J AU - Levy, S AU - Toga, AW AU - Smith, RD AU - Leahy, RM AU - Smith, DJ PY - 2007/08/20/ UR - http://dx.doi.org/10.1152/physiolgenomics.00287.2006 ER -