Extremely rapid induction of outer hair cell apoptosis in the chinchilla cochlea following exposure to impulse noise

@article{citeulike:834191, abstract = {We have reported the presence of OHC apoptosis and necrosis in the organ of Corti following exposure to intense noise. The current study was designed to investigate the rapidity and the initial pattern of outer hair cell (OHC) death induced by exposure to impulse noise. Chinchillas were exposed to 75 pairs of impulse noise at 155 dB peak sound pressure level presented over a time period of 75 s. At 5 or 30 min after the noise exposure, the cochleae were examined for morphological and biological indicators of apoptosis and necrosis. In the cochleae collected within 5 min after the 75-s noise exposure, there were clear signs of nuclear condensation and cell body shrinkage, suggesting the presence of OHC apoptosis. Apoptotic OHCs were further detected by positive staining of TUNEL and caspase-3 assays. In contrast to the rapid development of nuclear condensation, appearance of nuclear swelling, a necrotic phenotype, appeared at 30 min after the noise exposure. The results of the study demonstrate that induction of OHC apoptosis after the noise exposure is an extremely rapid process.}, author = {Hu, Bo H. and Henderson, Donald and Nicotera, Thomas M. }, citeulike-article-id = {834191}, doi = {10.1016/j.heares.2005.08.006}, journal = {Hearing Research}, keywords = {cochlea, noise}, month = {January}, number = {1-2}, pages = {16--25}, posted-at = {2006-09-07 23:28:24}, priority = {2}, title = {Extremely rapid induction of outer hair cell apoptosis in the chinchilla cochlea following exposure to impulse noise}, url = {http://dx.doi.org/10.1016/j.heares.2005.08.006}, volume = {211}, year = {2006} }

TY - JOUR ID - citeulike:834191 L3 - citeulike-article-id:834191 TI - Extremely rapid induction of outer hair cell apoptosis in the chinchilla cochlea following exposure to impulse noise JF - Hearing Research VL - 211 IS - 1-2 SP - 16 EP - 25 N2 - We have reported the presence of OHC apoptosis and necrosis in the organ of Corti following exposure to intense noise. The current study was designed to investigate the rapidity and the initial pattern of outer hair cell (OHC) death induced by exposure to impulse noise. Chinchillas were exposed to 75 pairs of impulse noise at 155 dB peak sound pressure level presented over a time period of 75 s. At 5 or 30 min after the noise exposure, the cochleae were examined for morphological and biological indicators of apoptosis and necrosis. In the cochleae collected within 5 min after the 75-s noise exposure, there were clear signs of nuclear condensation and cell body shrinkage, suggesting the presence of OHC apoptosis. Apoptotic OHCs were further detected by positive staining of TUNEL and caspase-3 assays. In contrast to the rapid development of nuclear condensation, appearance of nuclear swelling, a necrotic phenotype, appeared at 30 min after the noise exposure. The results of the study demonstrate that induction of OHC apoptosis after the noise exposure is an extremely rapid process. KW - cochlea KW - noise AU - Hu, Bo AU - Henderson, Donald AU - Nicotera, Thomas PY - 2006/01// UR - http://dx.doi.org/10.1016/j.heares.2005.08.006 ER -