Optical Saturation in Fluorescence Correlation Spectroscopy under Continuous-Wave and Pulsed Excitation

@article{citeulike:1095530, abstract = {A detailed theoretical and experimental study of the dependence of fluorescence correlation measurements on optical excitation power due to optical saturation effects is presented. It is shown that the sensitivity of a fluorescence correlation measurement on excitation power becomes increasingly stronger for decreasing excitation power. This makes exact measurements of diffusion coefficients with fluorescence correlation spectroscopy rather difficult. A strong difference of this behavior for continuous-wave and pulsed excitation is found.}, address = {Institute for Biological Information Processing 1, Forschungszentrum J\"{u}lich, 52425 J\"{u}lich, Germany, Fax: (+49) 2461-61-8069; Institute for Biological Information Processing 2, Forschungszentrum J\"{u}lich, 52425 J\"{u}lich, Germany}, author = {Gregor, Ingo and Patra, Digambara and Enderlein, J\"{o}rg }, citeulike-article-id = {1095530}, comment = {Sensitivity of FCS increase as excitation power decrease.}, doi = {10.1002/cphc.200400319}, journal = {ChemPhysChem}, keywords = {fcs, theory}, number = {1}, pages = {164--170}, posted-at = {2007-02-08 20:10:40}, priority = {1}, title = {Optical Saturation in Fluorescence Correlation Spectroscopy under Continuous-Wave and Pulsed Excitation}, url = {http://dx.doi.org/10.1002/cphc.200400319}, volume = {6}, year = {2005} }

TY - JOUR ID - citeulike:1095530 L3 - citeulike-article-id:1095530 TI - Optical Saturation in Fluorescence Correlation Spectroscopy under Continuous-Wave and Pulsed Excitation JF - ChemPhysChem VL - 6 IS - 1 SP - 164 EP - 170 AD - Institute for Biological Information Processing 1, Forschungszentrum Jülich, 52425 Jülich, Germany, Fax: (+49) 2461-61-8069; Institute for Biological Information Processing 2, Forschungszentrum Jülich, 52425 Jülich, Germany N2 - A detailed theoretical and experimental study of the dependence of fluorescence correlation measurements on optical excitation power due to optical saturation effects is presented. It is shown that the sensitivity of a fluorescence correlation measurement on excitation power becomes increasingly stronger for decreasing excitation power. This makes exact measurements of diffusion coefficients with fluorescence correlation spectroscopy rather difficult. A strong difference of this behavior for continuous-wave and pulsed excitation is found. KW - fcs KW - theory N1 - Sensitivity of FCS increase as excitation power decrease. AU - Gregor, Ingo AU - Patra, Digambara AU - Enderlein, Jörg PY - 2005/// UR - http://dx.doi.org/10.1002/cphc.200400319 ER -