Array-based electrical detection of DNA with nanoparticle probes.

@article{citeulike:532057, abstract = {A DNA array detection method is reported in which the binding of oligonucleotides functionalized with gold nanoparticles leads to conductivity changes associated with target-probe binding events. The binding events localize gold nanoparticles in an electrode gap; silver deposition facilitated by these nanoparticles bridges the gap and leads to readily measurable conductivity changes. An unusual salt concentration-dependent hybridization behavior associated with these nanoparticle probes was exploited to achieve selectivity without a thermal-stringency wash. Using this method, we have detected target DNA at concentrations as low as 500 femtomolar with a point mutation selectivity factor of approximately 100,000:1.}, address = {Department of Chemistry and Institute for Nanotechnology, Northwestern University, Evanston, IL 60208, USA.}, author = {Park, S. J. and Taton, T. A. and Mirkin, C. A. }, citeulike-article-id = {532057}, comment = {may be intersting}, doi = {10.1126/science.1067003}, issn = {1095-9203}, journal = {Science}, keywords = {array, dna, nanoparticle}, month = {February}, number = {5559}, pages = {1503--1506}, posted-at = {2007-02-05 18:39:58}, priority = {1}, title = {Array-based electrical detection of DNA with nanoparticle probes.}, url = {http://dx.doi.org/10.1126/science.1067003}, volume = {295}, year = {2002} }

TY - JOUR ID - citeulike:532057 L3 - citeulike-article-id:532057 TI - Array-based electrical detection of DNA with nanoparticle probes. JF - Science VL - 295 IS - 5559 SP - 1503 EP - 1506 AD - Department of Chemistry and Institute for Nanotechnology, Northwestern University, Evanston, IL 60208, USA. SN - 1095-9203 N2 - A DNA array detection method is reported in which the binding of oligonucleotides functionalized with gold nanoparticles leads to conductivity changes associated with target-probe binding events. The binding events localize gold nanoparticles in an electrode gap; silver deposition facilitated by these nanoparticles bridges the gap and leads to readily measurable conductivity changes. An unusual salt concentration-dependent hybridization behavior associated with these nanoparticle probes was exploited to achieve selectivity without a thermal-stringency wash. Using this method, we have detected target DNA at concentrations as low as 500 femtomolar with a point mutation selectivity factor of approximately 100,000:1. KW - array KW - dna KW - nanoparticle N1 - may be intersting AU - Park, SJ AU - Taton, TA AU - Mirkin, CA PY - 2002/02/22/ UR - http://dx.doi.org/10.1126/science.1067003 ER -