A High-Resolution Root Spatiotemporal Map Reveals Dominant Expression Patterns

@article{citeulike:1868819, abstract = {Transcriptional programs that regulate development are exquisitely controlled in space and time. Elucidating these programs that underlie development is essential to understanding the acquisition of cell and tissue identity. We present microarray expression profiles of a high-resolution set of developmental time points within a single Arabidopsis root and a comprehensive map of nearly all root cell types. These cell typespecific transcriptional signatures often predict previously unknown cellular functions. A computational pipeline identified dominant expression patterns that demonstrate transcriptional similarity between disparate cell types. Dominant expression patterns along the root's longitudinal axis do not strictly correlate with previously defined developmental zones, and in many cases, we observed expression fluctuation along this axis. Both robust co-regulation of gene expression and potential phasing of gene expression were identified between individual roots. Methods that combine these profiles demonstrate transcriptionally rich and complex programs that define Arabidopsis root development in both space and time. 10.1126/science.1146265}, author = {Brady, Siobhan M. and Orlando, David A. and Lee, Ji-Young and Wang, Jean Y. and Koch, Jeremy and Dinneny, Jose R. and Mace, Daniel and Ohler, Uwe and Benfey, Philip N. }, citeulike-article-id = {1868819}, doi = {http://dx.doi.org/10.1126/science.1146265}, journal = {Science}, keywords = {clustering, expression, fluorescent, microarray}, month = {November}, number = {5851}, pages = {801--806}, posted-at = {2007-11-05 14:32:26}, priority = {1}, title = {A High-Resolution Root Spatiotemporal Map Reveals Dominant Expression Patterns}, url = {http://dx.doi.org/10.1126/science.1146265}, volume = {318}, year = {2007} }

TY - JOUR ID - citeulike:1868819 L3 - citeulike-article-id:1868819 TI - A High-Resolution Root Spatiotemporal Map Reveals Dominant Expression Patterns JF - Science VL - 318 IS - 5851 SP - 801 EP - 806 N2 - Transcriptional programs that regulate development are exquisitely controlled in space and time. Elucidating these programs that underlie development is essential to understanding the acquisition of cell and tissue identity. We present microarray expression profiles of a high-resolution set of developmental time points within a single Arabidopsis root and a comprehensive map of nearly all root cell types. These cell typespecific transcriptional signatures often predict previously unknown cellular functions. A computational pipeline identified dominant expression patterns that demonstrate transcriptional similarity between disparate cell types. Dominant expression patterns along the root's longitudinal axis do not strictly correlate with previously defined developmental zones, and in many cases, we observed expression fluctuation along this axis. Both robust co-regulation of gene expression and potential phasing of gene expression were identified between individual roots. Methods that combine these profiles demonstrate transcriptionally rich and complex programs that define Arabidopsis root development in both space and time. 10.1126/science.1146265 KW - clustering KW - expression KW - fluorescent KW - microarray AU - Brady, Siobhan AU - Orlando, David AU - Lee, Ji-Young AU - Wang, Jean AU - Koch, Jeremy AU - Dinneny, Jose AU - Mace, Daniel AU - Ohler, Uwe AU - Benfey, Philip PY - 2007/11/02/ UR - http://dx.doi.org/10.1126/science.1146265 ER -