A genome-wide view of the spectrum of spontaneous mutations in yeast

@article{citeulike:2974440, abstract = {The mutation process ultimately defines the genetic features of all populations and, hence, has a bearing on a wide range of issues involving evolutionary genetics, inheritance, and genetic disorders, including the predisposition to cancer. Nevertheless, formidable technical barriers have constrained our understanding of the rate at which mutations arise and the molecular spectrum of their effects. Here, we report on the use of complete-genome sequencing in the characterization of spontaneously arising mutations in the yeast Saccharomyces cerevisiae. Our results confirm some findings previously obtained by indirect methods but also yield numerous unexpected findings, in particular a very high rate of point mutation and skewed distribution of base-substitution types in the mitochondrion, a very high rate of segmental duplication and deletion in the nuclear genome, and substantial deviations in the mutational profile among various model organisms.}, author = {Michael and Way and Krystalynne and Nicole and Christian and Erik and Joseph and Kazufusa and Shilpa and Daniel and Kelley}, citeulike-article-id = {2974440}, keywords = {genome-wide, mutation, yeast}, number = {27}, pages = {9272--9277}, posted-at = {2008-07-09 08:18:41}, priority = {2}, title = {A genome-wide view of the spectrum of spontaneous mutations in yeast}, url = {http://www.pnas.org/content/105/27/9272.full}, volume = {105}, year = {2008} }

TY - JOUR ID - citeulike:2974440 L3 - citeulike-article-id:2974440 TI - A genome-wide view of the spectrum of spontaneous mutations in yeast VL - 105 IS - 27 SP - 9272 EP - 9277 N2 - The mutation process ultimately defines the genetic features of all populations and, hence, has a bearing on a wide range of issues involving evolutionary genetics, inheritance, and genetic disorders, including the predisposition to cancer. Nevertheless, formidable technical barriers have constrained our understanding of the rate at which mutations arise and the molecular spectrum of their effects. Here, we report on the use of complete-genome sequencing in the characterization of spontaneously arising mutations in the yeast Saccharomyces cerevisiae. Our results confirm some findings previously obtained by indirect methods but also yield numerous unexpected findings, in particular a very high rate of point mutation and skewed distribution of base-substitution types in the mitochondrion, a very high rate of segmental duplication and deletion in the nuclear genome, and substantial deviations in the mutational profile among various model organisms. KW - genome-wide KW - mutation KW - yeast AU - Michael AU - Way AU - Krystalynne AU - Nicole AU - Christian AU - Erik AU - Joseph AU - Kazufusa AU - Shilpa AU - Daniel AU - Kelley PY - 2008/// UR - http://www.pnas.org/content/105/27/9272.full ER -